Gürcan M N, Koyutürk M, Yildiz H S, Cetin-Atalay R, Cetin A E
Bilkent University, Ankara, Turkey.
Biotechniques. 1999 Jun;26(6):1162-6, 1168-9. doi: 10.2144/99266bc01.
In polyacrylamide gel electrophoresis (PAGE) image analysis, it is important to determine the percentage of the protein of interest of a protein mixture. This study presents reliable computer software to determine this percentage. The region of interest containing the protein band is detected using the snake algorithm. The iterative snake algorithm is implemented in a multi-resolutional framework. The snake is initialized on a low-resolution image. Then, the final position of the snake at the low resolution is used as the initial position in the higher-resolution image. Finally, the area of the protein is estimated as the area enclosed by the final position of the snake.
在聚丙烯酰胺凝胶电泳（PAGE）图像分析中，确定蛋白质混合物中目标蛋白质的百分比很重要。本研究提出了用于确定该百分比的可靠计算机软件。使用蛇形算法检测包含蛋白质条带的感兴趣区域。迭代蛇形算法在多分辨率框架中实现。蛇形在低分辨率图像上初始化。然后，蛇形在低分辨率下的最终位置用作高分辨率图像中的初始位置。最后，将蛋白质的面积估计为蛇形最终位置所包围的面积。
