Chegini N, Rong H, Bennett B, Stone I K
Department of Obstetrics and Gynecology, University of Florida College of Medicine, Gainesville 32610-0294, USA.
J Soc Gynecol Investig. 1999 May-Jun;6(3):153-7. doi: 10.1016/s1071-5576(99)00007-6.
To determine the peritoneal fluid content of several cytokines and eicosanoids with inflammatory, anti-inflammatory, anti-fibrotic, and fibrotic activities, and to assess the relationship of these levels with the incidence of peritoneal adhesions.
Peritoneal fluids were collected from 30 subjects with adhesions (n = 22) or with normal pelvic anatomy (n = 8), and the level of interleukin-1 beta (IL-1 beta), tumor necrosis factor alpha (TNF-alpha), interleukin-10 (IL-10), interferon gamma (IFN-gamma), transforming growth factor beta-1 (TGF-beta 1), granulocyte macrophage-colony stimulating factor (GM-CSF), prostaglandin E2 (PGE2), leukotriene B4 (LTB4), and thromboxane B2 (TXB2) were determined by enzyme-linked immunosorbent assay (ELISA) and radioreceptor assay.
The peritoneal fluid content of these factors varied considerably, with low levels of IL-1 beta, TNF-alpha, IL-10, IFN-gamma, and GM-CSF. Only IFN-gamma levels were significantly lower in subjects with adhesions compared with the normal group (P < .05). The levels of total (latent + active) and active TGF-beta 1 were higher than those of other cytokines assayed and were significantly higher in subjects with adhesions compared with the normal group (P < .05). The peritoneal fluid content of PGE2, TXB2, and LTB4 was significantly higher than that of the cytokines and was higher, but not significantly so, in subjects with adhesions compared with normal subjects (P = .06).
Although the effect of length of time since the adhesions were formed is not known, the results indicate that peritoneal fluid content of these cytokines and eicosanoids, with the exception of IFN-gamma and TGF-beta 1, does not correlate with the presence of peritoneal adhesions.
测定具有炎症、抗炎、抗纤维化和纤维化活性的几种细胞因子和类花生酸的腹腔液含量，并评估这些水平与腹腔粘连发生率之间的关系。
从30名有粘连（n = 22）或盆腔解剖结构正常（n = 8）的受试者中收集腹腔液，采用酶联免疫吸附测定（ELISA）和放射受体测定法测定白细胞介素-1β（IL-1β）、肿瘤坏死因子α（TNF-α）、白细胞介素-10（IL-10）、干扰素γ（IFN-γ）、转化生长因子β-1（TGF-β1）、粒细胞巨噬细胞集落刺激因子（GM-CSF）、前列腺素E2（PGE2）、白三烯B4（LTB4）和血栓素B2（TXB2）的水平。
这些因子的腹腔液含量差异很大，IL-1β、TNF-α、IL-10、IFN-γ和GM-CSF水平较低。与正常组相比，粘连患者中只有IFN-γ水平显著降低（P <.05）。总（潜伏+活性）和活性TGF-β1水平高于其他检测的细胞因子，与正常组相比，粘连患者中的水平显著更高（P <.05）。PGE2、TXB2和LTB4的腹腔液含量显著高于细胞因子，与正常受试者相比，粘连患者中的含量更高，但差异不显著（P = 0.06）。
尽管粘连形成后的时间长度的影响尚不清楚，但结果表明，除IFN-γ和TGF-β1外，这些细胞因子和类花生酸的腹腔液含量与腹腔粘连的存在无关。
