用于在口腔螺旋体齿垢密螺旋体中表达异源基因的系统的开发及其在梅毒螺旋体flaA基因表达中的应用。
Development of a system for expressing heterologous genes in the oral spirochete Treponema denticola and its use in expression of the Treponema pallidum flaA gene.
作者信息
Chi B, Chauhan S, Kuramitsu H
机构信息
Department of Oral Biology, State University of New York at Buffalo, Buffalo, New York 14214, USA.
出版信息
Infect Immun. 1999 Jul;67(7):3653-6. doi: 10.1128/IAI.67.7.3653-3656.1999.
Abstract
The present communication describes the construction of a new Escherichia coli-Treponema denticola shuttle vector based on the naturally occurring spirochete plasmid pTS1 and the expression of the heterologous T. pallidum flaA gene from the plasmid in T. denticola. This new shuttle vector system should prove useful in characterizing virulence factors from unculturable pathogenic spirochetes.
摘要
本通讯描述了一种基于天然存在的螺旋体质粒pTS1构建的新型大肠杆菌-齿垢密螺旋体穿梭载体，以及该质粒中异源梅毒螺旋体flaA基因在齿垢密螺旋体中的表达。这种新型穿梭载体系统在鉴定不可培养的致病性螺旋体的毒力因子方面应会证明是有用的。
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