口腔螺旋体齿垢密螺旋体中的基因失活：flgE突变体的构建。
Gene inactivation in the oral spirochete Treponema denticola: construction of an flgE mutant.
作者信息
Li H, Ruby J, Charon N, Kuramitsu H
机构信息
Department of Oral Biology, State University of New York at Buffalo, 14214, USA.
出版信息
J Bacteriol. 1996 Jun;178(12):3664-7. doi: 10.1128/jb.178.12.3664-3667.1996.
Abstract
Treponema denticola is implicated in the etiology of periodontal diseases. We now report the construction of a specific flgE mutant of T. denticola ATCC 35405 following electroporation utilizing an erythromycin resistance cassette inserted into an flgE DNA fragment. The resulting mutant displays no visible motility and lacks periplasmic flagella as would be predicted from inactivation of the gene for the flagellar hook protein.
摘要
齿垢密螺旋体与牙周疾病的病因有关。我们现在报告，在利用插入flgE DNA片段的红霉素抗性盒进行电穿孔后，构建了齿垢密螺旋体ATCC 35405的特定flgE突变体。如鞭毛钩蛋白基因失活所预测的那样，所得突变体没有可见的运动性且缺乏周质鞭毛。
相似文献
1
Gene inactivation in the oral spirochete Treponema denticola: construction of an flgE mutant.口腔螺旋体齿垢密螺旋体中的基因失活：flgE突变体的构建。
J Bacteriol. 1996 Jun;178(12):3664-7. doi: 10.1128/jb.178.12.3664-3667.1996.
2
Insertional inactivation of Treponema denticola tap1 results in a nonmotile mutant with elongated flagellar hooks.齿垢密螺旋体tap1的插入失活导致了一个具有伸长鞭毛钩的无运动能力突变体。
J Bacteriol. 1999 Jun;181(12):3743-50. doi: 10.1128/JB.181.12.3743-3750.1999.
3
Genetic and biochemical analysis of the flagellar hook of Treponema phagedenis.溃蚀密螺旋体鞭毛钩的遗传与生化分析
J Bacteriol. 1994 Jun;176(12):3631-7. doi: 10.1128/jb.176.12.3631-3637.1994.
4
Relationship of Treponema denticola periplasmic flagella to irregular cell morphology.齿垢密螺旋体周质鞭毛与不规则细胞形态的关系。
J Bacteriol. 1997 Mar;179(5):1628-35. doi: 10.1128/jb.179.5.1628-1635.1997.
5
Complementation of a Treponema denticola flgE mutant with a novel coumermycin A1-resistant T. denticola shuttle vector system.利用新型抗香豆霉素A1的齿垢密螺旋体穿梭载体系统对齿垢密螺旋体flgE突变体进行互补。
Infect Immun. 2002 Apr;70(4):2233-7. doi: 10.1128/IAI.70.4.2233-2237.2002.
6
Homology of a plasmid from the spirochete Treponema denticola with the single-stranded DNA plasmids.来自齿垢密螺旋体的一种质粒与单链DNA质粒的同源性。
J Bacteriol. 1992 Apr;174(8):2724-8. doi: 10.1128/jb.174.8.2724-2728.1992.
7
Molecular characterization of a flagellar (fla) operon in the oral spirochete Treponema denticola ATCC 35405.口腔螺旋体齿垢密螺旋体ATCC 35405中鞭毛（fla）操纵子的分子特征分析
FEMS Microbiol Lett. 1999 Oct 1;179(1):31-6. doi: 10.1111/j.1574-6968.1999.tb08703.x.
8
The Role of Motility in Synergistic Biofilm Formation With .运动性在. 协同生物膜形成中的作用。
Front Cell Infect Microbiol. 2019 Dec 18;9:432. doi: 10.3389/fcimb.2019.00432. eCollection 2019.
9
Kanamycin Resistance Cassette for Genetic Manipulation of Treponema denticola.用于齿垢密螺旋体基因操作的卡那霉素抗性盒
Appl Environ Microbiol. 2015 Jul;81(13):4329-38. doi: 10.1128/AEM.00478-15. Epub 2015 Apr 17.
10
Conservation of msp, the gene encoding the major outer membrane protein of oral Treponema spp.口腔密螺旋体属主要外膜蛋白编码基因msp的保守性
J Bacteriol. 1997 Feb;179(4):1082-9. doi: 10.1128/jb.179.4.1082-1089.1997.
引用本文的文献
1
Emerging oral membrane proteins disorder neutrophil phosphoinositide signaling via phosphatidylinositol-4-phosphate 5-kinase.新兴的口腔膜蛋白通过磷脂酰肌醇-4-磷酸5-激酶扰乱中性粒细胞磷酸肌醇信号传导。
Front Oral Health. 2025 Apr 3;6:1568983. doi: 10.3389/froh.2025.1568983. eCollection 2025.
2
Dysbiosis linking periodontal disease and oral squamous cell carcinoma-A brief narrative review.将牙周病与口腔鳞状细胞癌联系起来的生态失调——一篇简要叙述性综述
Heliyon. 2024 May 31;10(11):e32259. doi: 10.1016/j.heliyon.2024.e32259. eCollection 2024 Jun 15.
3
Enhanced transformation efficiency in Treponema denticola enabled by SyngenicDNA-based plasmids lacking restriction-modification target motifs.基于缺失限制修饰靶序列的共生 DNA 质粒提高了密螺旋体（Treponema denticola）的转化效率。
Mol Oral Microbiol. 2023 Dec;38(6):455-470. doi: 10.1111/omi.12441. Epub 2023 Oct 25.
4
Approaches to Understanding Mechanisms of Dentilisin Protease Complex Expression in .理解牙蛋白酶复合物在……中表达机制的方法
Front Cell Infect Microbiol. 2021 May 18;11:668287. doi: 10.3389/fcimb.2021.668287. eCollection 2021.
5
Genetic Manipulations of Oral Spirochete Treponema denticola.口腔密螺旋体Treponema denticola 的遗传操作。
Methods Mol Biol. 2021;2210:15-23. doi: 10.1007/978-1-0716-0939-2_2.
6
Glutathione catabolism by Treponema denticola impacts its pathogenic potential.齿垢密螺旋体的谷胱甘肽分解代谢会影响其致病潜力。
Anaerobe. 2020 Apr;62:102170. doi: 10.1016/j.anaerobe.2020.102170. Epub 2020 Feb 7.
7
Roles of TroA and TroR in Metalloregulated Growth and Gene Expression in .TroA 和 TroR 在. 金属调控生长和基因表达中的作用
J Bacteriol. 2020 Mar 11;202(7). doi: 10.1128/JB.00770-19.
8
The Role of Motility in Synergistic Biofilm Formation With .运动性在. 协同生物膜形成中的作用。
Front Cell Infect Microbiol. 2019 Dec 18;9:432. doi: 10.3389/fcimb.2019.00432. eCollection 2019.
9
A pleiotropic role of FlaG in regulating the cell morphogenesis and flagellar homeostasis at the cell poles of Treponema denticola.FlaG 在调节栖牙髓卟啉单胞菌细胞极的细胞形态发生和鞭毛动态平衡中的多效作用。
Cell Microbiol. 2019 Feb;21(2):e12886. doi: 10.1111/cmi.12886. Epub 2018 Jul 23.
10
Activation of the Innate Immune System by Treponema denticola Periplasmic Flagella through Toll-Like Receptor 2.具核梭杆菌周质鞭毛通过Toll样受体2激活先天性免疫系统
Infect Immun. 2017 Dec 19;86(1). doi: 10.1128/IAI.00573-17. Print 2018 Jan.
本文引用的文献
1
Bacterial flagellar filaments and their component flagellins.细菌鞭毛丝及其组成成分鞭毛蛋白。
Can J Microbiol. 1993 May;39(5):451-72. doi: 10.1139/m93-066.
2
Bacterial mediators in periodontal disease.牙周疾病中的细菌介质
Clin Infect Dis. 1993 Jun;16 Suppl 4:S203-10. doi: 10.1093/clinids/16.supplement_4.s203.
3
Spirochaetes in oral infections.口腔感染中的螺旋体
Endod Dent Traumatol. 1993 Jun;9(3):87-94. doi: 10.1111/j.1600-9657.1993.tb00257.x.
4
Genetic and biochemical analysis of the flagellar hook of Treponema phagedenis.溃蚀密螺旋体鞭毛钩的遗传与生化分析
J Bacteriol. 1994 Jun;176(12):3631-7. doi: 10.1128/jb.176.12.3631-3637.1994.
5
Cloning and sequence analysis of a chymotrypsinlike protease from Treponema denticola.齿垢密螺旋体类胰凝乳蛋白酶样蛋白酶的克隆与序列分析
Infect Immun. 1994 Aug;62(8):3424-33. doi: 10.1128/iai.62.8.3424-3433.1994.
6
Virulence of a Porphyromonas gingivalis W83 mutant defective in the prtH gene.牙龈卟啉单胞菌W83中prtH基因缺陷型突变体的毒力
Infect Immun. 1995 Apr;63(4):1521-8. doi: 10.1128/iai.63.4.1521-1528.1995.
7
Evidence for two distinct locomotory phenotypes of Treponema denticola ATCC 35405.齿垢密螺旋体ATCC 35405两种不同运动表型的证据。
Oral Microbiol Immunol. 1995 Apr;10(2):122-4. doi: 10.1111/j.1399-302x.1995.tb00131.x.
8
Inactivation of Serpulina hyodysenteriae flaA1 and flaB1 periplasmic flagellar genes by electroporation-mediated allelic exchange.通过电穿孔介导的等位基因交换使猪痢疾蛇形螺旋体flaA1和flaB1周质鞭毛基因失活
J Bacteriol. 1995 Oct;177(20):5959-70. doi: 10.1128/jb.177.20.5959-5970.1995.
9
Treponema phagedenis has at least two proteins residing together on its periplasmic flagella.溃蚀性密螺旋体在其周质鞭毛上至少有两种蛋白质共存。
J Bacteriol. 1986 Apr;166(1):105-12. doi: 10.1128/jb.166.1.105-112.1986.
10
Quantitative relationship of Treponema denticola to severity of periodontal disease.齿垢密螺旋体与牙周病严重程度的定量关系。
Infect Immun. 1988 Apr;56(4):726-8. doi: 10.1128/iai.56.4.726-728.1988.
Zotero 插件