口腔螺旋体齿垢密螺旋体中的基因失活：flgE突变体的构建。
Gene inactivation in the oral spirochete Treponema denticola: construction of an flgE mutant.
作者信息
Li H, Ruby J, Charon N, Kuramitsu H
机构信息
Department of Oral Biology, State University of New York at Buffalo, 14214, USA.
出版信息
J Bacteriol. 1996 Jun;178(12):3664-7. doi: 10.1128/jb.178.12.3664-3667.1996.
Abstract
Treponema denticola is implicated in the etiology of periodontal diseases. We now report the construction of a specific flgE mutant of T. denticola ATCC 35405 following electroporation utilizing an erythromycin resistance cassette inserted into an flgE DNA fragment. The resulting mutant displays no visible motility and lacks periplasmic flagella as would be predicted from inactivation of the gene for the flagellar hook protein.
摘要
齿垢密螺旋体与牙周疾病的病因有关。我们现在报告，在利用插入flgE DNA片段的红霉素抗性盒进行电穿孔后，构建了齿垢密螺旋体ATCC 35405的特定flgE突变体。如鞭毛钩蛋白基因失活所预测的那样，所得突变体没有可见的运动性且缺乏周质鞭毛。
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本文引用的文献
1
Bacterial flagellar filaments and their component flagellins.
Can J Microbiol. 1993 May;39(5):451-72. doi: 10.1139/m93-066.
2
Bacterial mediators in periodontal disease.
Clin Infect Dis. 1993 Jun;16 Suppl 4:S203-10. doi: 10.1093/clinids/16.supplement_4.s203.
3
Spirochaetes in oral infections.
Endod Dent Traumatol. 1993 Jun;9(3):87-94. doi: 10.1111/j.1600-9657.1993.tb00257.x.
4
Genetic and biochemical analysis of the flagellar hook of Treponema phagedenis.
J Bacteriol. 1994 Jun;176(12):3631-7. doi: 10.1128/jb.176.12.3631-3637.1994.
5
Cloning and sequence analysis of a chymotrypsinlike protease from Treponema denticola.
Infect Immun. 1994 Aug;62(8):3424-33. doi: 10.1128/iai.62.8.3424-3433.1994.
6
Virulence of a Porphyromonas gingivalis W83 mutant defective in the prtH gene.
Infect Immun. 1995 Apr;63(4):1521-8. doi: 10.1128/iai.63.4.1521-1528.1995.
7
Evidence for two distinct locomotory phenotypes of Treponema denticola ATCC 35405.
Oral Microbiol Immunol. 1995 Apr;10(2):122-4. doi: 10.1111/j.1399-302x.1995.tb00131.x.
8
Inactivation of Serpulina hyodysenteriae flaA1 and flaB1 periplasmic flagellar genes by electroporation-mediated allelic exchange.
J Bacteriol. 1995 Oct;177(20):5959-70. doi: 10.1128/jb.177.20.5959-5970.1995.
9
Treponema phagedenis has at least two proteins residing together on its periplasmic flagella.
J Bacteriol. 1986 Apr;166(1):105-12. doi: 10.1128/jb.166.1.105-112.1986.
10
Quantitative relationship of Treponema denticola to severity of periodontal disease.
Infect Immun. 1988 Apr;56(4):726-8. doi: 10.1128/iai.56.4.726-728.1988.
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