Kaloyianni M, Trachana V, Boukla A
Zoology Department, School of Biology, Aristotle University of Thessaloniki, Greece.
Cell Biochem Funct. 1999 Jun;17(2):75-88. doi: 10.1002/(SICI)1099-0844(199906)17:2<75::AID-CBF807>3.0.CO;2-4.
In the presence of NH4Cl and hypotonic solutions, Rana balcanica red cells respond by increasing their volume. The stimulation of cellular volume by hypotonicity is more rapid than that of NH4Cl, while the maximum value is less than that observed in the presence of NH4Cl. Depending on the cause of swelling, (nct uptake of NH4Cl or decrease in external osmolality) cells show specific responses. The NH4Cl treatment causes a significant increase in intracellular Na+, from 5.14 +/- 0.78 to 29.84 +/- 0.47 mmoles l-1 cell, while hypotonicity leads to a significant decrease of this cation, to 3.85 +/- 0.25 mmoles l-1 cell in relation to the control, after 30 min of incubation of Rana balcanica erythrocytes. In addition, amiloiride significantly reverses the NH4Cl effect with respect to intracellular Na+. Both treatments cause a significant K+ loss in comparison with controls. Two glycolytic enzymes glyceraldehyde phosphate dehydrogenase (GAPDH) and pyruvate kinase (PK) of Rana balcanica haemolysate were found to respond to the NH4Cl effect by significantly decreasing their activity.
在氯化铵和低渗溶液存在的情况下，巴尔干林蛙红细胞会通过增加体积做出反应。低渗对细胞体积的刺激比氯化铵更快，但其最大值低于在氯化铵存在时观察到的值。根据肿胀的原因（氯化铵的净摄取或细胞外渗透压降低），细胞会表现出特定的反应。氯化铵处理会使细胞内钠离子显著增加，从5.14±0.78毫摩尔/升细胞增加到29.84±0.47毫摩尔/升细胞，而低渗会导致该阳离子显著减少，在巴尔干林蛙红细胞孵育30分钟后，相对于对照组，降至3.85±0.25毫摩尔/升细胞。此外，氨氯吡咪能显著逆转氯化铵对细胞内钠离子的影响。与对照组相比，两种处理都会导致显著的钾离子流失。发现巴尔干林蛙溶血产物中的两种糖酵解酶，即磷酸甘油醛脱氢酶（GAPDH）和丙酮酸激酶（PK），会通过显著降低其活性来响应氯化铵的作用。
