Zhang Y, LeRiche J C, Jackson S M, Garner D, Palcic B
Department of Radiation Oncology, Jiangsu Institute of Cancer Research, Nanjing, PR China.
Radiat Med. 1999 Jan-Feb;17(1):47-57.
DNA content and distribution in cell nuclei were studied in samples of fine-needle aspiration (FNA) from 27 locally advanced breast and head and neck cancers in two going randomized trials that compared accelerated fractionation to standard fractionation radiation in locally advanced breast cancer and head and neck cancer. Two image cytometry methods were compared: a new, fully automated DNA image cytometry system (AIC) and a conventional image cytometry (CIC) system with manual selection, focusing, and segmentation of cells. The results of both techniques were compared on the basis of DNA histogram parameters including DNA index (DI), mean DNA values (MDV), and Auer's DNA histogram patterns. An excellent correlation was achieved between the two imaging techniques in terms of DI (r=0.985, p<0.001) and MDV (r=0.951, p<0.001) as well as between Auer's histogram patterns, where both methods agreed completely. It was concluded in these analyses that the two image cytometry methods were equivalent. However, the AIC offered an advantage by scanning samples in a fully automated way, which represented significant time saving for cytopathologists working with the system, as well as a larger number of cells used in the automated analysis. With the automated image cytometer, 500 relevant cells were collected and analyzed in about 10 minutes, where with the interactive (manual) method, it took typically an hour to collect and analyze only about 250 cells. Seventeen samples were sufficient for flow analysis. Image cytometry and flow cytometry showed good agreement in DI determination; however, three cases reported as diploid by flow cytometry were found to be aneuploid by image cytometry techniques.
在两项正在进行的随机试验中，对27例局部晚期乳腺癌和头颈癌的细针穿刺（FNA）样本中的细胞核DNA含量及分布进行了研究。这两项试验比较了局部晚期乳腺癌和头颈癌中加速分割放疗与标准分割放疗的效果。比较了两种图像细胞术方法：一种新的全自动DNA图像细胞术系统（AIC）和一种传统的图像细胞术（CIC）系统，后者需人工选择、聚焦和分割细胞。基于DNA直方图参数，包括DNA指数（DI）、平均DNA值（MDV）和奥尔DNA直方图模式，对两种技术的结果进行了比较。两种成像技术在DI（r=0.985，p<0.001）和MDV（r=0.951，p<0.001）方面以及在奥尔直方图模式方面都具有极好的相关性，两种方法完全一致。这些分析得出的结论是，两种图像细胞术方法是等效的。然而，AIC的优势在于以全自动方式扫描样本，这为使用该系统的细胞病理学家节省了大量时间，并且在自动分析中使用的细胞数量更多。使用自动图像细胞仪，大约10分钟内可以收集并分析500个相关细胞，而使用交互式（手动）方法，通常需要一个小时才能收集并仅分析约250个细胞。17个样本足以进行流式分析。图像细胞术和流式细胞术在DI测定方面显示出良好的一致性；然而，通过流式细胞术报告为二倍体的3例病例，通过图像细胞术技术发现为非整倍体。
