Chen Y, Naito J
Division of Biofunctions Development, Graduate School of Bioagricultural Sciences, Nagoya University, Japan.
J Vet Med Sci. 1999 May;61(5):537-42. doi: 10.1292/jvms.61.537.
Classification of retinal ganglion cells (RGCs) in the chick central retina was studied by retrograde labeling of carbocyanine dye (DiI) and intracellular filling with Lucifer Yellow. Ganglion cells were divided into 4 groups, Group Ic/Is, Group IIc/IIs, Group IIIs, Group IVc, according to sizes of somal area and dendritic field and dendritic branching pattern. Group I cells had small somal area and small dendritic field. They were further divided into 2 subgroups by complexity (subgroup Ic) and simplicity (subgroup Is) of the dendritic arborization. Group II cells had medium-sized soma and dendritic field. They were also divided into subgroup IIc and IIs by the same definitions as those of subgroup Ic and Is. Group IIIs had medium-sized soma, large and simple dendritic arborization. Group IVc in which all cells had large soma, showed large and complex dendritic arborization. Cell populations of each group were 51.8% (subgroup Ic), 21.1% (subgroup Is), 6.2% (subgroup IIc), 14.6% (subgroup IIs), 4.2% (Group IIIs), and 2.1% (Group IVc). Subgroup Ic cells, which were very similar to beta-cells in the mammalian central area, represented about a half of the ganglion cell population. Cells in subgroup Is and IIs, which were not reported in the mammalian retina, were found in the chick central retina in relatively high population (35.7%). Morphological features of chick RGCs in the central retina were considered in comparison with those of other vertebrates.
通过花青染料（DiI）逆行标记和用鲁米诺黄进行细胞内填充，研究了鸡中枢视网膜中视网膜神经节细胞（RGCs）的分类。根据细胞体面积、树突野大小和树突分支模式，神经节细胞分为4组，即Ic/Is组、IIc/IIs组、IIIs组、IVc组。I组细胞的细胞体面积和树突野较小。根据树突分支的复杂程度（Ic亚组）和简单程度（Is亚组），它们又进一步分为2个亚组。II组细胞的细胞体和树突野中等大小。根据与Ic亚组和Is亚组相同的定义，它们也分为IIc亚组和IIs亚组。IIIs组细胞的细胞体中等大小，树突分支大且简单。IVc组所有细胞的细胞体都很大，其树突分支大且复杂。每组细胞的比例分别为51.8%（Ic亚组）、21.1%（Is亚组）、6.2%（IIc亚组）、14.6%（IIs亚组）、4.2%（IIIs组）和2.1%（IVc组）。与哺乳动物中枢区域的β细胞非常相似的Ic亚组细胞约占神经节细胞群体的一半。在哺乳动物视网膜中未报道过的Is亚组和IIs亚组细胞，在鸡中枢视网膜中以相对较高的比例（35.7%）被发现。通过与其他脊椎动物的比较，对鸡中枢视网膜中RGCs的形态特征进行了研究。
