通过白细胞滤除器去除克氏锥虫：一项电子显微镜研究。

Removal of Trypanosoma cruzi by white cell-reduction filters: an electronmicroscopic study.

作者信息

Fabron Júnior A, Bordin J O, Moraes-Souza H, FreyMüller E, Lages-Silva E

机构信息

Escola Paulista de Medicina da Universidade Federal de São Paulo, Brasil.

出版信息

Rev Soc Bras Med Trop. 1999 May-Jun;32(3):223-7. doi: 10.1590/s0037-86821999000300001.

DOI:10.1590/s0037-86821999000300001

PMID:10380559

Abstract

White cell (WBC)-reduction filters have been shown to be effective in removing infectious agents from infected blood products. In this study, the mechanisms of Trypanosoma cruzi (T. cruzi) retention by WBC-reduction filters were assessed. Human packed red blood cell (PRBC) and platelet concentrate (PC) samples were contaminated with T. cruzi organisms (Y strain; 3.4 x 10(6)/ml), and then filtered using WBC-reduction experimental filters that provided about 3 log10 WBC removal. Transmission electron microscopy sections showed that T. cruzi parasites were removed from contaminated PRBC and PC samples primarily by mechanical mechanism without interacting with filter fibers or blood cells. In addition, we found that T. cruzi parasites were also removed by a direct fiber adhesion. These data indicate that T. cruzi parasites are removed from infected blood not only by mechanical mechanism but also by biological mechanism probably mediated by parasite surface proteins.

摘要

白细胞（WBC）去除滤器已被证明在从受感染的血液制品中去除感染因子方面是有效的。在本研究中，评估了白细胞去除滤器截留克氏锥虫（T. cruzi）的机制。将人浓缩红细胞（PRBC）和血小板浓缩液（PC）样本用克氏锥虫生物体（Y株；3.4×10⁶/ml）污染，然后使用能去除约3个对数级白细胞的白细胞去除实验滤器进行过滤。透射电子显微镜切片显示，克氏锥虫寄生虫主要通过机械机制从受污染的PRBC和PC样本中被去除，而不与滤器纤维或血细胞相互作用。此外，我们发现克氏锥虫寄生虫也通过直接的纤维黏附被去除。这些数据表明，克氏锥虫寄生虫从受感染血液中被去除不仅通过机械机制，还可能通过由寄生虫表面蛋白介导的生物学机制。