一氧化氮是反硝化副球菌中NNR介导的转录激活的信号。
Nitric oxide is a signal for NNR-mediated transcription activation in Paracoccus denitrificans.
作者信息
Van Spanning R J, Houben E, Reijnders W N, Spiro S, Westerhoff H V, Saunders N
机构信息
Department of Molecular Cell Physiology, Faculty of Biology, BioCentrum Amsterdam, Vrije Universiteit, NL-1081 HV Amsterdam, The Netherlands.
出版信息
J Bacteriol. 1999 Jul;181(13):4129-32. doi: 10.1128/JB.181.13.4129-4132.1999.
Abstract
By using the 'lacZ gene, the activities of the nirI, nirS, and norC promoters were assayed in the wild type and in NNR-deficient mutants of Paracoccus denitrificans grown under various growth conditions. In addition, induction profiles of the three promoters in response to the presence of various nitrogenous oxides were determined. Transcription from the three promoters required the absence of oxygen and the presence both of the transcriptional activator NNR and of nitric oxide. The activity of the nnr promoter itself was halved after the cells had been switched from aerobic respiration to denitrification. This response was apparently not a result of autoregulation or of regulation by FnrP, since the nnr promoter was as active in the wild-type strain as it was in NNR- or FnrP-deficient mutants.
摘要
通过使用“lacZ基因，在不同生长条件下培养的反硝化副球菌野生型和NNR缺陷型突变体中检测了nirI、nirS和norC启动子的活性。此外，还测定了这三个启动子对各种氮氧化物存在的诱导谱。这三个启动子的转录需要无氧以及转录激活因子NNR和一氧化氮的存在。在细胞从有氧呼吸转换为反硝化作用后，nnr启动子自身的活性减半。这种反应显然不是自动调节或FnrP调节的结果，因为nnr启动子在野生型菌株中的活性与在NNR或FnrP缺陷型突变体中的活性相同。
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