铜对细菌一氧化二氮排放的控制及其对依赖维生素 B12 代谢的影响。
Copper control of bacterial nitrous oxide emission and its impact on vitamin B12-dependent metabolism.
机构信息
School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, United Kingdom.
出版信息
Proc Natl Acad Sci U S A. 2013 Dec 3;110(49):19926-31. doi: 10.1073/pnas.1314529110. Epub 2013 Nov 18.
Abstract
Global agricultural emissions of the greenhouse gas nitrous oxide (N2O) have increased by around 20% over the last 100 y, but regulation of these emissions and their impact on bacterial cellular metabolism are poorly understood. Denitrifying bacteria convert nitrate in soils to inert di-nitrogen gas (N2) via N2O and the biochemistry of this process has been studied extensively in Paracoccus denitrificans. Here we demonstrate that expression of the gene encoding the nitrous oxide reductase (NosZ), which converts N2O to N2, is regulated in response to the extracellular copper concentration. We show that elevated levels of N2O released as a consequence of decreased cellular NosZ activity lead to the bacterium switching from vitamin B12-dependent to vitamin B12-independent biosynthetic pathways, through the transcriptional modulation of genes controlled by vitamin B12 riboswitches. This inhibitory effect of N2O can be rescued by addition of exogenous vitamin B12.
摘要
过去 100 年来，全球农业排放的温室气体氧化亚氮（N2O）增加了约 20%，但对这些排放物的监管及其对细菌细胞代谢的影响仍知之甚少。反硝化细菌通过 N2O 将土壤中的硝酸盐转化为惰性的双氮气体（N2），这个过程的生物化学在 Paracoccus denitrificans 中已经得到了广泛研究。在这里，我们证明了编码一氧化二氮还原酶（NosZ）的基因的表达受到细胞外铜浓度的调节，NosZ 将 N2O 转化为 N2。我们表明，由于细胞 NosZ 活性降低而释放出的高水平 N2O，导致细菌从依赖维生素 B12 转向依赖维生素 B12 的生物合成途径，这是通过维生素 B12 核糖开关控制的基因的转录调控实现的。通过添加外源维生素 B12 可以挽救 N2O 的这种抑制作用。
相似文献
1
Copper control of bacterial nitrous oxide emission and its impact on vitamin B12-dependent metabolism.铜对细菌一氧化二氮排放的控制及其对依赖维生素 B12 代谢的影响。
Proc Natl Acad Sci U S A. 2013 Dec 3;110(49):19926-31. doi: 10.1073/pnas.1314529110. Epub 2013 Nov 18.
2
A Central Small RNA Regulatory Circuit Controlling Bacterial Denitrification and NO Emissions.一种调控细菌反硝化和 NO 排放的中心小 RNA 调控回路。
mBio. 2019 Aug 6;10(4):e01165-19. doi: 10.1128/mBio.01165-19.
3
Transcriptional and environmental control of bacterial denitrification and N2O emissions.细菌反硝化作用和一氧化二氮排放的转录及环境控制
FEMS Microbiol Lett. 2018 Mar 1;365(5). doi: 10.1093/femsle/fnx277.
4
is essential for whole-cell NO reduction in but not for assembly of copper centres of nitrous oxide reductase.对于完整细胞中 NO 的还原是必需的，但对于亚硝酸盐还原酶铜中心的组装则不是必需的。
Microbiology (Reading). 2020 Oct;166(10):909-917. doi: 10.1099/mic.0.000955.
5
Respiration and growth of R-1 with nitrous oxide as an electron acceptor.以氧化亚氮作为电子受体时 R-1 的呼吸和生长。
Microbiol Spectr. 2024 Jun 4;12(6):e0381123. doi: 10.1128/spectrum.03811-23. Epub 2024 Apr 22.
6
Effect of pH on the denitrification proteome of the soil bacterium Paracoccus denitrificans PD1222.pH 对土壤细菌脱氮假单胞菌 PD1222 脱氮蛋白组的影响。
Sci Rep. 2021 Aug 26;11(1):17276. doi: 10.1038/s41598-021-96559-2.
7
The impact of copper, nitrate and carbon status on the emission of nitrous oxide by two species of bacteria with biochemically distinct denitrification pathways.铜、硝酸盐和碳状况对具有不同生物化学反硝化途径的两种细菌排放一氧化二氮的影响。
Environ Microbiol. 2012 Jul;14(7):1788-800. doi: 10.1111/j.1462-2920.2012.02789.x. Epub 2012 May 30.
8
Nitrous Oxide Metabolism in Nitrate-Reducing Bacteria: Physiology and Regulatory Mechanisms.硝酸盐还原细菌中的一氧化二氮代谢：生理学与调控机制
Adv Microb Physiol. 2016;68:353-432. doi: 10.1016/bs.ampbs.2016.02.007. Epub 2016 Mar 29.
9
Modeling the effect of copper availability on bacterial denitrification.模拟铜供应对细菌反硝化作用的影响。
Microbiologyopen. 2013 Oct;2(5):756-65. doi: 10.1002/mbo3.111. Epub 2013 Jul 30.
10
Transient Accumulation of NO2- and N2O during Denitrification Explained by Assuming Cell Diversification by Stochastic Transcription of Denitrification Genes.通过假设反硝化基因的随机转录导致细胞多样化来解释反硝化过程中NO2-和N2O的瞬时积累。
PLoS Comput Biol. 2016 Jan 5;12(1):e1004621. doi: 10.1371/journal.pcbi.1004621. eCollection 2016 Jan.
引用本文的文献
1
Recent progress in microbial production and consumption of nitrous oxide in agricultural soils.农业土壤中一氧化二氮微生物产生与消耗的最新进展
World J Microbiol Biotechnol. 2025 Jun 27;41(7):235. doi: 10.1007/s11274-025-04464-x.
2
Selective enrichment of high-affinity clade II NO-reducers in a mixed culture.在混合培养物中对高亲和力的II类一氧化氮还原菌进行选择性富集。
ISME Commun. 2025 Feb 5;5(1):ycaf022. doi: 10.1093/ismeco/ycaf022. eCollection 2025 Jan.
3
Metabolic labour division trade-offs in denitrifying microbiomes.反硝化微生物群落中的代谢分工权衡
ISME J. 2025 Jan 2;19(1). doi: 10.1093/ismejo/wraf020.
4
Soil microbiome interventions for carbon sequestration and climate mitigation.用于碳固存和缓解气候的土壤微生物群落干预措施。
mSystems. 2025 Jan 21;10(1):e0112924. doi: 10.1128/msystems.01129-24. Epub 2024 Dec 18.
5
Copper pyrazole addition regulates soil mineral nitrogen turnover by mediating microbial traits.添加吡唑铜通过介导微生物特性来调节土壤矿质氮周转。
Front Microbiol. 2024 Oct 1;15:1433816. doi: 10.3389/fmicb.2024.1433816. eCollection 2024.
6
H-NOX Influences Biofilm Formation, Central Metabolism, and Quorum Sensing in .H-NOX 影响. 中的生物膜形成、中心代谢和群体感应。
J Proteome Res. 2024 Nov 1;23(11):4988-5000. doi: 10.1021/acs.jproteome.4c00466. Epub 2024 Oct 6.
7
Unraveling the genetic potential of nitrous oxide reduction in wastewater treatment: insights from metagenome-assembled genomes.揭示废水处理中氧化亚氮还原的遗传潜力：宏基因组组装基因组的启示。
Appl Environ Microbiol. 2024 Sep 18;90(9):e0217723. doi: 10.1128/aem.02177-23. Epub 2024 Aug 13.
8
pH selects for distinct NO-reducing microbiomes in tropical soil microcosms.pH值在热带土壤微观世界中选择出不同的硝酸盐还原微生物群落。
ISME Commun. 2024 May 8;4(1):ycae070. doi: 10.1093/ismeco/ycae070. eCollection 2024 Jan.
9
Sustained bacterial NO reduction at acidic pH.在酸性 pH 下持续的细菌 NO 还原。
Nat Commun. 2024 May 15;15(1):4092. doi: 10.1038/s41467-024-48236-x.
10
Genomic and environmental controls on Castellaniella biogeography in an anthropogenically disturbed subsurface.人为干扰地下环境中卡氏杆菌生物地理学的基因组和环境控制因素
Environ Microbiome. 2024 Apr 26;19(1):26. doi: 10.1186/s40793-024-00570-9.
本文引用的文献
1
A riboswitch-regulated antisense RNA in Listeria monocytogenes.李斯特菌中受核糖体开关调控的反义 RNA
Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13132-7. doi: 10.1073/pnas.1304795110. Epub 2013 Jul 22.
2
Unexpected nondenitrifier nitrous oxide reductase gene diversity and abundance in soils.土壤中出乎意料的非硝化亚硝酸盐还原酶基因多样性和丰度。
Proc Natl Acad Sci U S A. 2012 Nov 27;109(48):19709-14. doi: 10.1073/pnas.1211238109. Epub 2012 Nov 12.
3
B12 cofactors directly stabilize an mRNA regulatory switch.B12 辅因子直接稳定一个 mRNA 调节开关。
Nature. 2012 Dec 6;492(7427):133-7. doi: 10.1038/nature11607. Epub 2012 Oct 14.
4
The impact of copper, nitrate and carbon status on the emission of nitrous oxide by two species of bacteria with biochemically distinct denitrification pathways.铜、硝酸盐和碳状况对具有不同生物化学反硝化途径的两种细菌排放一氧化二氮的影响。
Environ Microbiol. 2012 Jul;14(7):1788-800. doi: 10.1111/j.1462-2920.2012.02789.x. Epub 2012 May 30.
5
Novel inducers of the envelope stress response BaeSR in Salmonella Typhimurium: BaeR is critically required for tungstate waste disposal.沙门氏菌中包膜应激反应 BaeSR 的新型诱导物：钨酸盐废物处理对 BaeR 有严格的要求。
PLoS One. 2011;6(8):e23713. doi: 10.1371/journal.pone.0023713. Epub 2011 Aug 23.
6
N2O binding at a [4Cu:2S] copper-sulphur cluster in nitrous oxide reductase.一氧化二氮结合在亚硝基还原酶的一个[4Cu:2S]铜-硫簇中。
Nature. 2011 Aug 14;477(7363):234-7. doi: 10.1038/nature10332.
7
The evolution and future of Earth's nitrogen cycle.地球氮循环的演化与未来。
Science. 2010 Oct 8;330(6001):192-6. doi: 10.1126/science.1186120.
8
Nitrous oxide (N2O): the dominant ozone-depleting substance emitted in the 21st century.一氧化二氮（N₂O）：21世纪排放的主要消耗臭氧层物质。
Science. 2009 Oct 2;326(5949):123-5. doi: 10.1126/science.1176985. Epub 2009 Aug 27.
9
Mitigating release of the potent greenhouse gas N(2)O from the nitrogen cycle - could enzymic regulation hold the key?减轻氮循环中强效温室气体一氧化二氮（N₂O）的排放——酶调节会是关键所在吗？
Trends Biotechnol. 2009 Jul;27(7):388-97. doi: 10.1016/j.tibtech.2009.03.009. Epub 2009 Jun 3.
10
Multiple posttranscriptional regulatory mechanisms partner to control ethanolamine utilization in Enterococcus faecalis.多种转录后调控机制协同作用，以控制粪肠球菌中乙醇胺的利用。
Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4435-40. doi: 10.1073/pnas.0812194106. Epub 2009 Feb 25.
Zotero 插件