培养的大脑神经元中由一种蛋白质因子诱导的突触增强。
Synaptic potentiation induced by a protein factor in cultured cerebral neurons.
作者信息
Kudoh S N, Kiyosue K, Kasai M, Taguchi T
机构信息
Department of Organic Materials, Osaka National Research Institute, Ikeda, Japan.
出版信息
Cell Mol Neurobiol. 1999 Oct;19(5):575-85. doi: 10.1023/a:1006976200474.
Abstract
- We reported in a previous paper that long-lasting enhancement of spontaneous excitatory post synaptic currents (SEPSCs) in cultured chick cerebral neurons was induced by exposure to a conditioned medium (CM) prepared by Mg(2+)-free treatment of neurons. This suggested that the CM contained a diffusible factor(s) for the potentiation. 2. In this paper, the factor(s) was shown to be a protein(s) by heat and trypsin treatment of the CM. 3. The factor induced the potentiation within 5 min, but it was not required for maintenance of increased SEPSCs. 4. The factors in CM induced the potentiation without protein synthesis. 5. Protein synthesis at least in postsynaptic neurons, was indispensable to induce the potentiation by the Mg(2+)-free condition.
摘要
- 我们在之前的一篇论文中报道，通过将神经元用无镁处理制备的条件培养基（CM）处理培养的鸡脑神经元，可诱导自发兴奋性突触后电流（SEPSCs）的长期增强。这表明CM中含有一种用于增强作用的可扩散因子。2. 在本文中，通过对CM进行加热和胰蛋白酶处理，表明该因子是一种蛋白质。3. 该因子在5分钟内诱导增强作用，但维持SEPSCs增加则不需要它。4. CM中的因子在无蛋白质合成的情况下诱导增强作用。5. 至少突触后神经元中的蛋白质合成对于在无镁条件下诱导增强作用是必不可少的。
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本文引用的文献
1
Neurotrophins and time: different roles for TrkB signaling in hippocampal long-term potentiation.神经营养因子与时间：TrkB信号在海马体长期增强效应中的不同作用
Neuron. 1997 Sep;19(3):653-64. doi: 10.1016/s0896-6273(00)80378-5.
2
Long-lasting enhancement of synaptic activity in dissociated cerebral neurons induced by brief exposure to Mg2+-free conditions.短暂暴露于无镁离子环境下诱导的离体脑神经元突触活动的长期增强。
Neurosci Res. 1997 Aug;28(4):337-44. doi: 10.1016/s0168-0102(97)00061-8.
3
Propagation of activity-dependent synaptic depression in simple neural networks.简单神经网络中活动依赖型突触抑制的传播
Nature. 1997 Jul 31;388(6641):439-48. doi: 10.1038/41267.
4
Selective formation of silent synapses on immature postsynaptic cells in cocultures of chick neurons of different ages.
Brain Res Dev Brain Res. 1997 Apr 18;99(2):201-7. doi: 10.1016/s0165-3806(97)00008-4.
5
Strong activation of parallel fibers produces localized calcium transients and a form of LTD that spreads to distant synapses.平行纤维的强烈激活会产生局部钙瞬变以及一种会扩散到远处突触的长时程抑制形式。
Neuron. 1996 Mar;16(3):601-10. doi: 10.1016/s0896-6273(00)80079-3.
6
Two modes of activity-dependent synaptogenesis of cerebral neurons in vitro.体外培养的脑神经元依赖活动的两种突触发生模式。
Neuroreport. 1996 Feb 29;7(3):701-4. doi: 10.1097/00001756-199602290-00005.
7
Synapse formation in dissociated cell cultures of embryonic chick cerebral neurons.胚胎鸡脑神经元解离细胞培养中的突触形成。
Brain Res Dev Brain Res. 1993 Jul 16;74(1):146-50. doi: 10.1016/0165-3806(93)90096-s.
8
Glial contributions to excitatory neurotransmission in cultured hippocampal cells.胶质细胞对培养海马细胞兴奋性神经传递的作用。
Nature. 1994 Mar 3;368(6466):59-62. doi: 10.1038/368059a0.
9
Long-lasting neurotrophin-induced enhancement of synaptic transmission in the adult hippocampus.成年海马体中神经营养因子诱导的突触传递的持久增强。
Science. 1995 Mar 17;267(5204):1658-62. doi: 10.1126/science.7886457.
10
Long-lasting potentiation of synaptic transmission in the dentate area of the anaesthetized rabbit following stimulation of the perforant path.在麻醉兔的齿状区，刺激穿通通路后突触传递的长时程增强。
J Physiol. 1973 Jul;232(2):331-56. doi: 10.1113/jphysiol.1973.sp010273.
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