Kawasaki S, Oshitani H, Suzuki H, Arakawa M, Mizuta K, Imaizumi M, Tsuchiya S, Konno T
Department of Public Health, Niigata University School of Medicine, Niigata, Japan.
Microbiol Immunol. 1999;43(4):359-64. doi: 10.1111/j.1348-0421.1999.tb02416.x.
In order to investigate the longitudinal molecular epidemiology of cytomegalovirus (CMV) infections associated with bone marrow transplantation (BMT) in Japanese children, we analyzed 36 CMV strains from 11 cases. Three regions (DNA polymerase, glycoprotein H, and immediate-early regions) of CMV DNA were amplified by polymerase chain reaction (PCR), and amplified products were each digested with two restriction enzymes, followed by electrophoresis. These restriction fragment length polymorphism (RFLP) analyses allowed the differentiation of 36 strains into 13 genotypes. Each patient excreted his or her own CMV with distinct genotype over the study period of up to one year. CMVs of two different genotypes were recovered during a one-month study from one recipient, who received a peripheral blood stem cell transplantation. Although the majority of patients and donors were CMV-seropositive before BMT, multiple CMV infections might not be common and the reactivation of latently infected CMV might be prominent in Japanese children receiving transplants.
为了研究日本儿童骨髓移植（BMT）相关的巨细胞病毒（CMV）感染的纵向分子流行病学，我们分析了11例患者的36株CMV毒株。通过聚合酶链反应（PCR）扩增CMV DNA的三个区域（DNA聚合酶、糖蛋白H和即刻早期区域），扩增产物分别用两种限制性内切酶消化，然后进行电泳。这些限制性片段长度多态性（RFLP）分析可将36株毒株分为13种基因型。在长达一年的研究期间，每位患者排出的都是具有独特基因型的自身CMV。在一名接受外周血干细胞移植的受者为期一个月的研究中，发现了两种不同基因型的CMV。虽然大多数患者和供者在BMT前CMV血清学呈阳性，但多重CMV感染可能并不常见，在接受移植的日本儿童中，潜伏感染的CMV再激活可能较为突出。
