Bunting M, Bernstein K E, Greer J M, Capecchi M R, Thomas K R
Hematology Division, Department of Internal Medicine, University of Utah, Salt Lake City, Utah 84112, USA.
Genes Dev. 1999 Jun 15;13(12):1524-8. doi: 10.1101/gad.13.12.1524.
A procedure is described that directs the self-induced deletion of DNA sequences as they pass through the male germ line of mice. The testes-specific promoter from the angiotensin-converting enzyme gene was used to drive expression of the Cre-recombinase gene. Cre was linked to the selectable marker Neor, and the two genes flanked with loxP elements. This cassette was targeted to the Hoxa3 gene in mouse ES cells that were in turn used to generate chimeric mice. In these chimeras, somatic cells derived from the ES cells retained the cassette, but self-excision occurred in all ES-cell-derived sperm.
本文描述了一种方法,可在DNA序列通过小鼠雄性生殖系时引导其自我诱导缺失。使用来自血管紧张素转换酶基因的睾丸特异性启动子来驱动Cre重组酶基因的表达。Cre与可选择标记Neor相连,这两个基因侧翼带有loxP元件。该盒式结构靶向小鼠胚胎干细胞中的Hoxa3基因,这些胚胎干细胞随后被用于生成嵌合小鼠。在这些嵌合体中,源自胚胎干细胞的体细胞保留了该盒式结构,但所有源自胚胎干细胞的精子中都发生了自我切除。