一种从透翅榕蛾（Perina nuda，Fabricius）中分离出的新型类微小核糖核酸病毒，即PnPV。

A new picorna-like virus, PnPV, isolated from ficus transparent wing moth, Perina nuda (Fabricius).

作者信息

Wang C H, Wu C Y, Lo C F

机构信息

Department of Entomology, National Taiwan University, Taipei, 107, Taiwan, Republic of China.

出版信息

J Invertebr Pathol. 1999 Jul;74(1):62-8. doi: 10.1006/jipa.1999.4842.

DOI:10.1006/jipa.1999.4842

PMID:10388548

Abstract

Two viruses, Perina nuda nucleopolyhedrovirus and a new picorna-like virus, were previously isolated from P. nuda larvae with flacherie. In this study the new picorna-like virus was characterized using physical and biochemical methods. This small virus appears to belong to the family Picornaviridae and we propose the name PnPV. PnPV can be propagated in its homogenous cell line, NTU-PN-HH. PnPV purified from the cell line resembles PnPV isolated from insects: under electron microscopy, it exhibits icosahedral symmetry, measures 30 nm in diameter, and has no envelope and no distinct surface structure in negatively stained preparations. In addition, we show here that PnPV has a buoyant density of 1.381 g/ml in cesium chloride, the viral genome was composed of one single-stranded RNA molecule with a length of 10 kb, and poly(A) tract and polyacrylamide gel electrophoresis of purified viral particles revealed three major (31.5, 29.7, and 28.4 kDa) and three minor (27. 0, 24.5, and 4.0 kDa) structural proteins.

摘要

此前，从患有软化病的樟蚕幼虫中分离出了两种病毒，即樟蚕核多角体病毒和一种新的类微小核糖核酸病毒。在本研究中，利用物理和生化方法对这种新的类微小核糖核酸病毒进行了特性分析。这种小型病毒似乎属于微小核糖核酸病毒科，我们提议将其命名为樟蚕痘病毒（PnPV）。PnPV能够在其同源细胞系NTU-PN-HH中增殖。从该细胞系中纯化得到的PnPV与从昆虫中分离出的PnPV相似：在电子显微镜下，它呈现二十面体对称，直径为30纳米，在负染制剂中无包膜且无明显表面结构。此外，我们在此表明，PnPV在氯化铯中的浮力密度为1.381克/毫升，病毒基因组由一个长度为10千碱基的单链RNA分子组成，纯化病毒颗粒的聚腺苷酸尾和聚丙烯酰胺凝胶电泳显示有三种主要（31.5、29.7和28.4千道尔顿）和三种次要（27.0、24.5和4.0千道尔顿）结构蛋白。