Lery X, Fediere G, Taha A, Salah M, Giannotti J
Entomovirology Laboratory ORSTOM, Cairo, Giza code 12211, Egypt.
J Invertebr Pathol. 1997 Jan;69(1):7-13. doi: 10.1006/jipa.1996.4617.
A persistent infection in a Galleria mellonella cell line was revealed when infected with a maize stem borer picorna-like virus isolated on Sesamia cretica (MSBV). The new virus, completely different from the MSBV, is designated as G. mellonella cell line virus (GmclV), induces spectacular cytopathic effects, and is also considered efficient in vivo. The GmclV is a 29-nm-diameter isometric virus, with single-strand RNA of 2.9 x 10(6) Da molecular weight with a poly(A) tract. Its capsid is constituted of only two major polypeptides, of 34,500 and 32,500 Da, and no minor bands could be detected. The characteristics of the GmclV do not permit us to classify it with assurance. Even though it has not yet been identified as a picornavirus, it can be classified in the small RNA virus group of the Picornaviridae. G. mellonella represents a very interesting model, owing to the fact that two different persistent viruses belonging to the same family were isolated in vivo and in vitro, to further the understanding of the general phenomenon of persistency and induction.
用从埃及蛀茎夜蛾（Sesamia cretica）分离得到的玉米螟类小核糖核酸病毒（MSBV）感染大蜡螟（Galleria mellonella）细胞系时，发现了一种持续性感染。这种与MSBV完全不同的新病毒被命名为大蜡螟细胞系病毒（GmclV），它能引发显著的细胞病变效应，并且在体内也被认为是有效的。GmclV是一种直径为29纳米的等轴病毒，具有分子量为2.9×10⁶道尔顿的单链RNA以及一个聚腺苷酸尾。它的衣壳仅由两种主要多肽组成，分子量分别为34500道尔顿和32500道尔顿，未检测到次要条带。GmclV的特征使我们无法确切地对其进行分类。尽管它尚未被鉴定为小核糖核酸病毒，但可归类于小核糖核酸病毒科的小RNA病毒组。大蜡螟是一个非常有趣的模型，因为在体内和体外都分离出了属于同一科的两种不同的持续性病毒，这有助于进一步理解持续性和诱导的一般现象。
