克林沙星对氟喹诺酮耐药西班牙临床分离株的体外活性

In vitro activity of clinafloxacin against fluoroquinolone resistant Spanish clinical isolates.

作者信息

López-Brea M, Prieto N, Domingo D, de las Cuevas C, Sánchez I, Alarcón T

机构信息

Department of Microbiology, Hospital Universitario de la Princesa, Madrid, Spain.

出版信息

Int J Antimicrob Agents. 1999 Jun;12(1):33-9. doi: 10.1016/s0924-8579(99)00054-0.

DOI:10.1016/s0924-8579(99)00054-0

PMID:10389645

Abstract

The in vitro activity of clinafloxacin against 162 ciprofloxacin-resistant clinical isolates was determined. Isolates were selected when their MIC to ciprofloxacin was 2 mg/l (intermediate) or > 2 mg/l (resistant). The following strains were tested: 61 Escherichia coli, 12 Klebsiella pneumoniae, 7 Proteus mirabilis, 21 Serratia marcescens, 4 Enterobacter cloacae, 21 Pseudomonas aeruginosa, 21 Staphylococcus. aureus (resistant to methicillin) and 15 Enterococcus spp. Clinafloxacin, ciprofloxacin, ofloxacin and norfloxacin activities were evaluated by agar dilution using Müeller-Hinton agar according to NCCLS recommendations. Of the 162 isolates, 16 (9.8%) were intermediate and 146 (90.1%) resistant to ciprofloxacin. 95 of the 162 strains (58.6%) were susceptible, 27 (16.7%) intermediately susceptible, and 40 strains (24.7%) were resistant to clinafloxacin. The percentage susceptible to clinafloxacin was 65.6% for E. coli, 75% for K. pneumoniae, 71.4% for P. mirabilis, 28.6% for S. marcescens, 75% for E. cloacae, 33.3% for P. aeruginosa, 90.5% for S. aureus and 40% for Enterococcus spp. Clinafloxacin was active against 58.6% of the ciprofloxacin-resistant clinical isolates tested. It was particularly active against S. aureus strains resistant to both ciprofloxacin and methicillin.

摘要

测定了克林沙星对162株耐环丙沙星临床分离株的体外活性。当分离株对环丙沙星的MIC为2mg/L（中介）或>2mg/L（耐药）时进行选择。测试了以下菌株：61株大肠埃希菌、12株肺炎克雷伯菌、7株奇异变形杆菌、21株粘质沙雷菌、4株阴沟肠杆菌、21株铜绿假单胞菌、21株金黄色葡萄球菌（耐甲氧西林）和15株肠球菌属。根据NCCLS建议，使用Müeller-Hinton琼脂通过琼脂稀释法评估克林沙星、环丙沙星、氧氟沙星和诺氟沙星的活性。在162株分离株中，16株（9.8%）对环丙沙星中介，146株（90.1%）耐药。162株菌株中有95株（58.6%）对克林沙星敏感，27株（16.7%）中介敏感，40株（24.7%）对克林沙星耐药。克林沙星的敏感率在大肠埃希菌中为65.6%，在肺炎克雷伯菌中为75%，在奇异变形杆菌中为71.4%，在粘质沙雷菌中为28.6%，在阴沟肠杆菌中为75%，在铜绿假单胞菌中为33.3%，在金黄色葡萄球菌中为90.5%，在肠球菌属中为40%。克林沙星对58.6%的测试耐环丙沙星临床分离株有活性。它对同时耐环丙沙星和甲氧西林的金黄色葡萄球菌菌株特别有活性。