Andrew S E, Hsiao L, Milhausen K, Jirik F R
Department of Medical Genetics, Medical Sciences Building, University of Alberta, Edmonton, AB, Canada.
Mutat Res. 1999 Jun 30;427(2):89-97. doi: 10.1016/s0027-5107(99)00094-9.
We have compared the spontaneous mutation frequency and spectrum of lacI genes recovered from a rat embryonic fibroblast line transfected with a lambda-phage shuttle vector (Rat2lambdalacI) using both the traditional plaque assay as well as a positive selection assay. In addition, mutation frequencies and spectrum were determined after treatment of the cells with either the intracellular superoxide-generating compound, menadione, or UVC light. The differences in mutation frequency between the two systems suggested that the selectable assay was better at discerning relatively small mutation frequency increases, more rapidly and at lower cost, than the plaque assay method. Some novel lacI mutations were observed in mutants derived from the selectable assay. This indicates that the selectable assay system may be a useful tool for assessing the mutagenic potential of different agents.
我们使用传统的噬菌斑测定法以及阳性选择测定法，比较了从转染了λ噬菌体穿梭载体（Rat2lambdalacI）的大鼠胚胎成纤维细胞系中回收的lacI基因的自发突变频率和谱。此外，在用细胞内超氧化物生成化合物甲萘醌或紫外线照射处理细胞后，测定了突变频率和谱。两种系统之间突变频率的差异表明，与噬菌斑测定法相比，选择测定法在辨别相对较小的突变频率增加方面更好，速度更快且成本更低。在源自选择测定法的突变体中观察到了一些新的lacI突变。这表明选择测定系统可能是评估不同试剂诱变潜力的有用工具。
