Ho T F, Yang B S, Huang Y T, Hung C T
Department of Medical Technology, Chungtai Institute of Health Science and Technology, Taichung, Republic of China.
Vox Sang. 1999;76(4):226-30. doi: 10.1159/000031056.
For years, blood transfusion centers in Taiwan have used the Quantitative Buffy Coat (QBC(R)) Hematology System for platelet counts on capillary blood samples in the laboratory screening of apheresis donors. The system has not been evaluated for the prediction of yields in plateletpheresis. Methods : The QBC instrument was evaluated for reproducibility of platelet counts and compared with five electronic cell counters. We also collected both capillary and venous blood from voluntary donors before donation and counted platelets, comparing the QBC system and an electronic blood cell counter (Sysmex K1000). The correlation between donors' predonation platelet counts and plateletpheresis yields was analyzed.
The R values for platelet counts between the QBC Hematology System and other electronic counters are lower (0.759-0. 890) than among the electronic counters (0.929-0.973). The mean capillary platelet count and the mean venous platelet count were 241. 9+/-50.3x10(3)/microl and 233.2 +/-47.9x10(3)/microl by the QBC system, and 244.9+/-54.1x10(3)/microl and 218.9+/-46.5x10(3)/microl by the Sysmex K1000, respectively. Linear regression analysis showed that platelet yields correlated well with donors' predonation platelet counts using the Sysmex K1000 counter (R = 0.777- 0.890, p<0.001), but not with the QBC system (R = 0.326 approximately 0.755, p<0.05).
The QBC Hematology System is not accurate enough to determine predonation platelet counts that are to be used for calculating the number of processing cycles for plateletpheresis.
多年来，台湾的输血中心在单采献血者的实验室筛查中一直使用定量血沉棕黄层（QBC®）血液学系统对毛细血管血样进行血小板计数。该系统尚未针对血小板单采的产量预测进行评估。方法：对QBC仪器进行血小板计数重复性评估，并与五台电子细胞计数器进行比较。我们还在自愿献血者献血前采集了毛细血管血和静脉血，并对血小板进行计数，比较了QBC系统和电子血细胞计数器（Sysmex K1000）。分析了献血者献血前血小板计数与血小板单采产量之间的相关性。
QBC血液学系统与其他电子计数器之间血小板计数的R值（0.759 - 0.890）低于电子计数器之间的R值（0.929 - 0.973）。QBC系统测得的平均毛细血管血小板计数和平均静脉血小板计数分别为241.9±50.3×10³/微升和233.2±47.9×10³/微升，Sysmex K1000测得的分别为244.9±54.1×10³/微升和218.9±46.5×10³/微升。线性回归分析表明，使用Sysmex K1000计数器时血小板产量与献血者献血前血小板计数相关性良好（R = 0.777 - 0.890，p<0.001），但与QBC系统无关（R = 0.326至0.755，p<0.05）。
QBC血液学系统在确定用于计算血小板单采处理周期数的献血前血小板计数方面不够准确。
