G蛋白对溶解的人透明带介导的顶体反应和透明带结合的调节作用。
G-protein regulation of the solubilized human zona pellucida-mediated acrosome reaction and zona pellucida binding.
作者信息
Bastiaan H, Franken D, Wranz P
机构信息
Department of Obstetrics & Gynaecology, University of Stellenbosch, Tygerberg Hospital, South Africa.
出版信息
J Assist Reprod Genet. 1999 Jul;16(6):332-6. doi: 10.1023/a:1020462201291.
PURPOSE
The study aimed to evaluate the (i) regulatory role of Gi-like protein during the acrosome reaction (AR) of normal sperm donors and (ii) the role of intact acrosomes during sperm-zona binding.
METHODS
The acrosomal exocytosis of spermatozoa incubated with solubilized zona pellucida (ZP) at a final concentration of 1 ZP/microliter was compared with 10 microM calcium ionophore A23187 and 30% (v/v) pooled human follicular fluid (HFF). Spermatozoa were incubated with 1, 10, and 100 ng/ml pertussis toxin (PT) during capacitation to functionally inactivate the Gi-like protein. The sperm-zona binding potential of 100 ng/ml PT-treated spermatozoa followed, by exposure to 1 ZP/microliter, revealed significantly higher zona-bound spermatozoa compared to controls treated with 1 ZP/microliter only.
RESULTS
PT treatment of spermatozoa did not affect sperm motility, however, inhibited the percentage AR induced by solubilized ZP. In contrast, the A23187- and HFF-induced ARs were not sensitive to PT treatment. PT inhibition of the ZP-induced AR occurred in a concentration-dependent manner, with maximal effects observed at 100 ng/ml PT.
CONCLUSIONS
In conclusion, it seems that PT-sensitive Gi-like protein in human spermatozoa plays an important regulatory role in the AR induced by the human ZP, and this underlines the importance of intact acrosomes during sperm-zona binding.
目的
本研究旨在评估（i）Gi样蛋白在正常精子供体顶体反应（AR）过程中的调节作用，以及（ii）完整顶体在精子与透明带结合过程中的作用。
方法
将精子与终浓度为1 ZP/微升的溶解透明带（ZP）孵育后的顶体胞吐作用，与10微摩尔钙离子载体A23187和30%（v/v）人卵泡液（HFF）进行比较。在获能过程中，将精子与1、10和100纳克/毫升百日咳毒素（PT）孵育，以功能性灭活Gi样蛋白。随后，将100纳克/毫升PT处理的精子暴露于1 ZP/微升，结果显示与仅用1 ZP/微升处理的对照组相比，与透明带结合的精子显著增多。
结果
PT处理精子不影响精子活力，但抑制了溶解ZP诱导的AR百分比。相反，A23187和HFF诱导的AR对PT处理不敏感。PT对ZP诱导的AR的抑制呈浓度依赖性，在100纳克/毫升PT时观察到最大效应。
结论
总之，人类精子中对PT敏感的Gi样蛋白似乎在人ZP诱导的AR中起重要调节作用，这突出了完整顶体在精子与透明带结合过程中的重要性。
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