Cross N L, Morales P, Overstreet J W, Hanson F W
Department of Obstetrics and Gynecology, School of Medicine, University of California, Davis 95616.
Biol Reprod. 1988 Feb;38(1):235-44. doi: 10.1095/biolreprod38.1.235.
We have used two approaches to test the ability of the human zona pellucida to induce acrosome reactions in human sperm. First, nonviable human oocytes were incubated for 1 min in a suspension of capacitated sperm (of which fewer than 5% were acrosome-reacted) to allow binding of about 200 sperm per oocyte. Some of the oocytes were fixed immediately, and the remainder were fixed after a further 1-h incubation without free-swimming sperm. As determined by light microscopy, sperm on the zona were only 3 +/- 2% (avg. +/- SD) acrosome-reacted at 1 min, and the incidence increased to 46 +/- 15% during the next hour. Electron microscopy confirmed that most sperm on the zona at 1 min were acrosome-intact. A few sperm were in an early stage of the acrosome reaction. Acrosome reactions occurring on the zona during the subsequent hour appeared to be morphologically normal. Second, treatment of sperm in suspension with acid-disaggregated zonae (2 to 4 zonae/microliter) increased the incidence of acrosome-reacted sperm from 3 +/- 1% to 24 +/- 4%. We conclude that the human zona pellucida, or material intimately associated with it, can induce acrosome reactions in human sperm.
我们采用了两种方法来测试人透明带诱导人精子发生顶体反应的能力。第一，将无活力的人卵母细胞在获能精子悬液（顶体反应的精子少于5%）中孵育1分钟，使每个卵母细胞结合约200个精子。部分卵母细胞立即固定，其余的在无游离游动精子的情况下再孵育1小时后固定。通过光学显微镜观察，在1分钟时，位于透明带上的精子只有3±2%（平均值±标准差）发生顶体反应，在接下来的1小时内，这一发生率增至46±15%。电子显微镜证实，在1分钟时，位于透明带上的大多数精子顶体完整。少数精子处于顶体反应的早期阶段。在随后1小时内发生于透明带上的顶体反应在形态上似乎是正常的。第二，用酸解聚的透明带（2至4个透明带/微升）处理精子悬液，使发生顶体反应的精子发生率从3±1%增至24±4%。我们得出结论，人透明带或与之紧密相关的物质能够诱导人精子发生顶体反应。
