Lockaby S B, Hoerr F J, Kleven S H, Lauerman L H
Department of Veterinary Pathobiology, Auburn University, AL 36849, USA.
Avian Dis. 1999 Apr-Jun;43(2):331-7.
Pathogenicity of Mycoplasma synoviae (MS) was examined in specific-pathogen-free (SPF) white leghorn chicken embryos. Six isolates of MS were inoculated into 7-day embryos via the yolk sac. Isolates were evaluated for gross and microscopic lesions through 19 days' incubation and for embryo lethality through 20 days' incubation. Isolates in decreasing order of lethality, from lowest to highest 50% embryo lethal dose, were WVU 1853, K1968, K1858, FMT, 92D8034, and F10-2AS. Embryo lethality was consistent with lesion incidence and severity. Embryo lethality did not correlate with previous results regarding pathogenicity of these same six isolates in SPF broiler chickens.
在无特定病原体(SPF)的白来航鸡胚中检测了滑膜支原体(MS)的致病性。将6株MS分离株通过卵黄囊接种到7日龄鸡胚中。通过19天的孵育评估分离株的大体和微观病变,并通过20天的孵育评估胚胎致死率。按照致死率从低到高(50%胚胎致死剂量)的顺序,分离株依次为WVU 1853、K1968、K1858、FMT、92D8034和F10-2AS。胚胎致死率与病变发生率和严重程度一致。胚胎致死率与之前关于这6株相同分离株在SPF肉鸡中的致病性结果不相关。