Scott D B, Jin W, Ledford H K, Jung H S, Honma M A
Developmental, Cell, and Molecular Biology Group/Department of Botany, Duke University, Durham, North Carolina 27708-1000, USA.
Plant Physiol. 1999 Jul;120(3):675-84. doi: 10.1104/pp.120.3.675.
We have identified a new locus that regulates vegetative phase change and flowering time in Arabidopsis. An early-flowering mutant, eaf1 (early flowering 1) was isolated and characterized. eaf1 plants flowered earlier than the wild type under either short-day or long-day conditions, and showed a reduction in the juvenile and adult vegetative phases. When grown under short-day conditions, eaf1 plants were slightly pale green and had elongated petioles, phenotypes that are observed in mutants altered in either phytochrome or the gibberellin (GA) response. eaf1 seed showed increased resistance to the GA biosynthesis inhibitor paclobutrazol, suggesting that GA metabolism and/or response had been altered. Comparison of eaf1 to other early-flowering mutants revealed that eaf1 shifts to the adult phase early and flowers early, similarly to the phyB (phytochrome B) and spy (spindly) mutants. eaf1 maps to chromosome 2, but defines a locus distinct from phyB, clf (curly leaf), and elf3 (early-flowering 3). These results demonstrate that eaf1 defines a new locus involved in an autonomous pathway and may affect GA regulation of flowering.
我们已经鉴定出一个调控拟南芥营养生长阶段转变和开花时间的新位点。分离并鉴定了一个早花突变体eaf1（early flowering 1）。在短日或长日条件下，eaf1植株比野生型开花早，并且其幼年期和成年营养期缩短。在短日条件下生长时，eaf1植株呈浅绿色，叶柄伸长，这些表型在光敏色素或赤霉素（GA）反应发生改变的突变体中也有观察到。eaf1种子对GA生物合成抑制剂多效唑的抗性增强，表明GA代谢和/或反应发生了改变。将eaf1与其他早花突变体进行比较发现，与phyB（光敏色素B）和spy（spindly）突变体类似，eaf1提前进入成年期并提前开花。eaf1定位于第2号染色体，但定义了一个与phyB、clf（卷曲叶）和elf3（早花3）不同的位点。这些结果表明，eaf1定义了一个参与自主途径的新位点，并且可能影响GA对开花的调控。
