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[The optimization of a method for determining RNAse activity by using high-polymer RNA].

作者信息

Kolpakov A I, Il'inskaia O N

出版信息

Klin Lab Diagn. 1999 May(5):14-6.

Abstract

Different methods for precipitation of free nucleotides are compared with the aim of improving the method for estimating RNAse activity. The precipitators were 0.75% uranyl acetate in 0.25% HClO4, 96% ethanol with MgCl2 in different concentrations, pure ethanol, and chlorine acid. Measurements of RNAse activity by means of 0.02M MgCl2 in 96% ethanol whose volume is equal to the reaction mixture were the optimal.

摘要

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