Lissi E A, Modak B, Torres R, Escobar J, Urzua A
Facultad de Química y Biología, Departamento de Ciencias Químicas, Universidad de Santiago de Chile.
Free Radic Res. 1999 Jun;30(6):471-7. doi: 10.1080/10715769900300511.
Total reactive antioxidant potential (TRAP) of resinous exudates from Heliotropium species was evaluated by measuring the bleaching of stable free radicals. The antioxidant capacity of the resinous exudates in Trolox equivalents, evaluated from the bleaching of ABTS derived radical cations, ranged from 2.0 M (H. huascoense) to 5.2 M (H. stenophyllum), indicating a very high concentration of phenolic compounds. Considerably smaller values were obtained by measuring the bleaching of DPPH radicals. The ratio between the values obtained employing ABTS derived radicals and DPPH, ranged from 37 (H. megalanthum) to 4.5 (H. chenopodiaceum variety typica). The magnitude of the difference can be considered as an indication of the relative reactivity of the antioxidants present in the exudates. Similar ratios were observed when stoichiometric coefficients were evaluated for representative purified flavonoids obtained from the resinous exudates.
通过测量稳定自由基的褪色情况，评估了天芥菜属植物树脂渗出物的总反应性抗氧化潜力（TRAP）。根据ABTS衍生自由基阳离子的褪色情况评估，树脂渗出物以Trolox当量计的抗氧化能力范围为2.0 M（瓦斯科天芥菜）至5.2 M（狭叶天芥菜），表明酚类化合物浓度非常高。通过测量DPPH自由基的褪色情况获得的值要小得多。使用ABTS衍生自由基和DPPH获得的值之间的比率范围为37（大花天芥菜）至4.5（藜叶天芥菜变种典型变种）。差异的大小可被视为渗出物中存在的抗氧化剂相对反应性的指标。当对从树脂渗出物中获得的代表性纯化黄酮类化合物评估化学计量系数时，观察到了类似的比率。
