Gergely A, Hegedús H, Horváth P, Noszál B, Szász G, Zsila F
SOTE Gyógyszerészi Kémiai Intézet, Budapest.
Acta Pharm Hung. 1999 Jun;69(3):128-34.
Chiroptical spectroscopy, and its double and triple hyphenated combinations with UV-VIS spectroscopy and/or separation techniques constitute great progress in pharmaceutical analysis, of which five recently developed methods are surveyed here, as follows: The determination of enantiomeric purity by double, CD/UV detection without, and with the latter one provides enhanced sensitivity and selectivity. The determination of chromatographic peak homogeneity, by double detection, and peak slicing by recording. The separation, identification and four stereoisomers of doubly chiral compounds, by HPLC separation on chiral column, and optical-chiroptical detection.
手性光谱及其与紫外可见光谱和/或分离技术的双重和三重联用技术在药物分析领域取得了巨大进展，本文综述了最近开发的五种方法，具体如下：通过双重检测、无紫外检测的圆二色光谱/紫外检测以及有紫外检测的圆二色光谱/紫外检测来测定对映体纯度，后者可提高灵敏度和选择性。通过双重检测测定色谱峰的同质性，并通过记录进行峰切割。通过在手性柱上进行高效液相色谱分离和光-手性光谱检测，对双 chiral 化合物的四种立体异构体进行分离、鉴定。 （注：原文中“doubly chiral compounds”的“doubly”表述可能有误，推测可能是“bis-chiral compounds”之类的表述，暂按原文翻译）
