Utsumi T, Mizuta T, Fujii Y, Shiono H, Okumura M, Minami M, Takeda S, Miyoshi S, Matsuda H
First Department of Surgery, Osaka University Medical School, Japan.
Transplantation. 1999 Jun 27;67(12):1622-6. doi: 10.1097/00007890-199906270-00019.
We reported the increased nitric oxide (NO) level in exhaled air of rat lung transplant recipients during acute rejection (AR). The aim of this study was to determine the site and level of NO production in the rejected graft.
Rat lung transplantation was performed in isografts and allografts.
In isografts, no AR and no significant increase in NO production was identified. In allografts, grades I-II of AR was seen on postoperative day (POD) 3 and grade III on POD 5. NO produced by BAL cells increased on both POD 3 (11.8+/-2.0 parts per billion [ppb]) and POD 5 (115.3+/-66.9 ppb). There was a highly significant correlation between the level of NO and the severity of AR (p=0.862, P<0.005). BAL cells from allografts expressed iNOS mRNA. Among them, macrophages, lymphocytes and neutrophils were immunostained for iNOS.
NO produced by BAL cells was detected in the early stages of rejection. Therefore, it may serve as a sensitive indicator of AR in lung transplantation.
我们报道了大鼠肺移植受者在急性排斥反应（AR）期间呼出气体中一氧化氮（NO）水平升高。本研究的目的是确定排斥移植物中NO产生的部位和水平。
在同基因移植和异基因移植中进行大鼠肺移植。
在同基因移植中，未发现急性排斥反应，NO产生也无显著增加。在异基因移植中，术后第3天出现I-II级急性排斥反应，术后第5天出现III级。支气管肺泡灌洗（BAL）细胞产生的NO在术后第3天（11.8±2.0十亿分之一[ppb]）和术后第5天（115.3±66.9 ppb）均增加。NO水平与急性排斥反应的严重程度之间存在高度显著的相关性（p = 0.862，P < 0.005）。来自异基因移植的BAL细胞表达诱导型一氧化氮合酶（iNOS）mRNA。其中，巨噬细胞、淋巴细胞和中性粒细胞进行了iNOS免疫染色。
在排斥反应的早期阶段检测到BAL细胞产生的NO。因此，它可能作为肺移植中急性排斥反应的敏感指标。
