Gabellec M M, Crumeyrolle-Arias M, Le Saux F, Auriou N, Jacque C, Haour F
Unite de Pharmacologie NeuroImmuno-Endocriniennne, Institut Pasteur, Paris, France.
Neurosci Res. 1999 Apr;33(4):251-60. doi: 10.1016/s0168-0102(99)00014-0.
In order to evaluate the role of IL-1 production in post-traumatic brain, transcripts for IL-1 (alpha, beta, RA) have been quantified following RT-PCR, in hippocampus and cortex after injury of either hippocampus (Hip) or striatum (Stri). Moreover, 125I IL-1alpha binding sites have been directly quantified using binding experiments on brain sections and quantitative autoradiography. Under basal conditions, levels of PCR products were very low. On day 1, IL-1RA transcripts only were strongly increased in the hippocampus after Hip-lesions and in cortex after Stri lesion. Transcripts were back to control values on day 7 post-lesion. IL-1 receptor densities in the hippocampus (dentate gyrus) were decreased at day 1 around the site of the lesion (but not on the contralateral side) and were back to controls on day 7 indicating a transient and local IL-1 production in the surroundings of the lesion. No changes were found following Stri lesion. This study provides further evidence of the role of the IL-1 molecules family, notably IL-1RA, in the brain reaction to trauma.
为了评估白细胞介素-1（IL-1）的产生在创伤性脑损伤中的作用，在海马体（Hip）或纹状体（Stri）损伤后，通过逆转录聚合酶链反应（RT-PCR）对海马体和皮质中IL-1（α、β、RA）的转录本进行了定量分析。此外，利用脑切片结合实验和定量放射自显影技术直接对125I IL-1α结合位点进行了定量分析。在基础条件下，聚合酶链反应（PCR）产物水平非常低。在损伤后第1天，仅IL-1RA转录本在海马体损伤后的海马体中以及纹状体损伤后的皮质中显著增加。损伤后第7天，转录本恢复到对照值。海马体（齿状回）中的IL-1受体密度在损伤后第1天在损伤部位周围降低（但对侧未降低），并在第7天恢复到对照水平，表明在损伤周围存在短暂的局部IL-1产生。纹状体损伤后未发现变化。这项研究进一步证明了IL-1分子家族，尤其是IL-1RA，在大脑对创伤的反应中的作用。
