尿液作为聚合酶链反应（PCR）中模板DNA的另一个潜在来源。
Urine as another potential source for template DNA in polymerase chain reaction (PCR).
作者信息
Hayakawa S, Takenaka O
机构信息
Department of Ecology and Social Behavior, Primate Research Institute, Kyoto University, Aichi-ken, Japan.
出版信息
Am J Primatol. 1999;48(4):299-304. doi: 10.1002/(SICI)1098-2345(1999)48:4<299::AID-AJP5>3.0.CO;2-G.
This technical note examines the potential for preparing template DNA in polymerase chain reactions (PCR) from urine in Japanese macaques (Macaca fuscata). Microsatellite band patterns from urine samples showed close agreement with those of blood and fecal samples, and only a few hundred microl of urine yielded a template DNA for PCR. This research will increase the opportunity for scientists to examine the genetic backgrounds of their target animals by using non-invasive sample collection in the wild.
本技术说明探讨了从日本猕猴（Macaca fuscata）尿液中制备聚合酶链反应（PCR）模板DNA的可能性。尿液样本的微卫星条带模式与血液和粪便样本的条带模式高度一致，仅几百微升尿液就能产生用于PCR的模板DNA。这项研究将增加科学家们通过在野外进行非侵入性样本采集来研究目标动物遗传背景的机会。
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