González-Muñoz M, Gárate T, Puente S, Subirats M, Moneo I
Centro de Investigación Clínica e Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain.
Am J Trop Med Hyg. 1999 Jun;60(6):974-9. doi: 10.4269/ajtmh.1999.60.974.
The host immune response in onchocerciasis is believed to contribute to the clinical manifestations of infection. Mazzotti and chronic inflammatory reactions might be mediated by mechanisms involving specific IgE and reactivity of mast cells and basophils to the parasite antigens. In this report, we show that Onchocerca volvulus antigens are capable of inducing histamine release. Three types of extracts were prepared from the parasite: soluble total, surface, and cuticular collagen. Soluble extracts released histamine in all individuals with onchocerciasis at significantly higher levels (P < 0.05) than those found in endemic controls, but similar levels to those found in patients with mansonellosis. However, cuticular collagen induced significantly (P < 0.01) higher histamine release in patients with onchocerciasis than in those with mansonellosis. No reactivity against human type IV collagen was observed. Implications derived from the presence of sensitized basophils in the pathogenesis of onchocerciasis are discussed.
盘尾丝虫病中的宿主免疫反应被认为与感染的临床表现有关。马佐蒂反应和慢性炎症反应可能由涉及特异性IgE以及肥大细胞和嗜碱性粒细胞对寄生虫抗原反应性的机制介导。在本报告中，我们表明盘尾丝虫抗原能够诱导组胺释放。从该寄生虫制备了三种类型的提取物：可溶性总提取物、表面提取物和表皮胶原蛋白提取物。可溶性提取物在所有盘尾丝虫病患者中释放组胺的水平显著高于（P < 0.05）流行区对照者，但与曼氏丝虫病患者中的水平相似。然而，表皮胶原蛋白在盘尾丝虫病患者中诱导的组胺释放显著高于（P < 0.01）曼氏丝虫病患者。未观察到对人IV型胶原蛋白的反应性。讨论了致敏嗜碱性粒细胞在盘尾丝虫病发病机制中的意义。
