Wojciechowska J A, Lewitin E, Revina L P, Zalunin I A, Chestukhina G G
Laboratory of Protein Chemistry, Institute for Microbial Genetics, Moscow, Russia.
FEBS Lett. 1999 Jun 18;453(1-2):46-8. doi: 10.1016/s0014-5793(99)00650-x.
Genes cry26Aal and cry28Aal were cloned from Bacillus thuringiensis ssp. finitimus strain B-1166 VKPM. This strain forms insecticidal crystal bodies either outside or inside the exosporium. The deduced amino acid sequence of the cry26Aal gene product included seven residues determined to be an N-terminal part of a chymotrypsin-treated delta-endotoxin isolated from the same strain. Earlier this protein was detected in both free and spore-associated types of crystals [Revina et al., Biokhimia (1999) in press]. Neither BtI nor BtII promoter sequences were found upstream of the open reading frames in both genes. Southern hybridization has shown that the surroundings of both genes at least 3 kb upstream and downstream of the open reading frames are unique. We suggest that the protein Cry26Aal in both types of crystal bodies is synthesized under the control of one and the same genomic locus.
cry26Aal和cry28Aal基因是从苏云金芽孢杆菌有限亚种菌株B-1166 VKPM中克隆得到的。该菌株在芽孢外壁的内部或外部形成杀虫晶体。cry26Aal基因产物推导的氨基酸序列包含7个残基，这些残基被确定为从同一菌株中分离出的经胰凝乳蛋白酶处理的δ-内毒素的N端部分。此前在游离型和孢子相关型晶体中均检测到了这种蛋白质[Revina等人，《生物化学》（1999年）待发表]。在这两个基因的开放阅读框上游均未发现BtI和BtII启动子序列。Southern杂交表明，两个基因开放阅读框上下游至少3 kb的区域是独特的。我们认为，两种类型晶体中的Cry26Aal蛋白是在同一个基因组位点的控制下合成的。
