Lindh C H, Jönsson B A, Johannesson G, Zhang X D, Welinder H, Brittebo E B
Department of Occupational and Environmental Medicine, Institute of Laboratory Medicine, University Hospital, Lund, Sweden.
Toxicology. 1999 Jun 15;134(2-3):153-68. doi: 10.1016/s0300-483x(99)00032-3.
Hexahydrophthalic anhydride (HHPA; CAS No. 13149-00-3) is a highly allergenic compound commonly used in the chemical industry. Guinea pigs and rats were exposed to [3H2]HHPA by inhalation for 3-8 h and were killed at various intervals during 7 days. The tissue distribution of non-volatile and covalently bound radioactivity was studied by autoradiography. Tissue bound radioactivity was mainly found in the mucosa of the upper respiratory airways, whereas negligible levels were observed in the lungs. In addition, tissue bound radioactivity was present in the gastrointestinal tract and conjunctiva. Moreover, in the cortex of the kidneys in rats, but not in guinea pigs, a low level of tissue bound radioactivity was found. The radioactivity in the tissues persisted for at least 7 days after the end of exposure. Plasma proteins and soluble proteins from trachea, lung, and kidney from [3H2]HHPA-exposed animals were separated by gel filtration. The radioactivity in dialysed plasma was mainly found in the same fractions as albumin. The soluble proteins from trachea, lung, and kidney in both rats and guinea pigs showed a similar pattern as found in blood. The radioactivity in dialysed plasma from both guinea pigs and rats seemed to decay according to a two-compartment model. The non-extractable binding of [3H2]HHPA in the upper respiratory airways and conjunctiva may be of relevance for symptoms in workers with allergy, since they mainly develop symptoms and signs from the nose and eyes.
六氢邻苯二甲酸酐（HHPA；化学物质登记号：13149 - 00 - 3）是一种常用于化学工业的高致敏性化合物。豚鼠和大鼠通过吸入[³H₂]HHPA暴露3 - 8小时，并在7天内的不同时间点处死。通过放射自显影研究了非挥发性和共价结合放射性的组织分布。组织结合放射性主要存在于上呼吸道黏膜，而在肺部观察到的水平可忽略不计。此外，在胃肠道和结膜中也存在组织结合放射性。而且，在大鼠肾脏皮质中发现了低水平的组织结合放射性，而豚鼠中未发现。暴露结束后，组织中的放射性至少持续7天。对暴露于[³H₂]HHPA的动物的血浆蛋白以及气管、肺和肾脏的可溶性蛋白进行凝胶过滤分离。透析血浆中的放射性主要存在于与白蛋白相同的组分中。大鼠和豚鼠气管、肺和肾脏的可溶性蛋白显示出与血液中相似的模式。豚鼠和大鼠透析血浆中的放射性似乎按照二室模型衰减。[³H₂]HHPA在上呼吸道和结膜中的不可提取结合可能与过敏工人的症状有关，因为他们主要从鼻子和眼睛出现症状和体征。
