McIntosh M, Bao H, Lee C
Department of Nutrition and Foodservice Systems, School of Human Environmental Sciences, University of North Carolina at Greensboro, Greensboro, North Carolina 27402-6170, USA.
Proc Soc Exp Biol Med. 1999 Jul;221(3):198-206. doi: 10.1046/j.1525-1373.1999.d01-77.x.
The purpose of this study was to determine the impact of dehydroepiandrosterone (DHEA) and corticosterone (CORT) treatment, using implants as a route of administration, on specific hormones, metabolites, and enzymes involved in energy metabolism. Sixty male Sprague-Dawley rats, 325 g initial weight, were implanted subcutaneously for 3 weeks with time-release pellets containing either DHEA or CORT at doses of 0, 10, 25, 50, or 100 mg in this 2 x 5 factorial experiment. In general, body weights and food intakes decreased as the level of steroid hormones increased. In contrast to DHEA treatment, rats receiving the 50- and 100-mg doses of CORT had lighter thymus glands and spleens and heavier epididymal and retroperitoneal fat pads than their controls. Rats treated with 100 mg of DHEA had lowered serum levels of triglycerides and lipid hydroperoxides whereas rats treated with 100 mg of CORT had higher levels of these blood lipids compared to their respective controls. In contrast to DHEA treatment, there was a dose-dependent increase in liver lipid content and the specific activities of the hepatic lipogenic enzymes glucose-6-phosphate dehydrogenase, malic enzyme, and fatty acid synthase in response to CORT treatment. Rats treated with 100 mg of DHEA had higher serum levels of IGF-1 than control rats. Conversely, rats treated with 100 mg of CORT had lower serum levels of IGF-1 and higher serum levels of testosterone, progesterone, and insulin than their controls. These data demonstrate the lipogenic actions of corticosterone in rats. Conversely, DHEA treatment reduced serum and hepatic lipids. Furthermore, these data suggest that using implants instead of bolus injections of steroids may be a more physiological approach for studying the influence of these steroids on lipid metabolism.
本研究的目的是确定使用植入物作为给药途径的脱氢表雄酮（DHEA）和皮质酮（CORT）治疗对能量代谢中特定激素、代谢物和酶的影响。在这个2×5析因实验中，将60只初始体重为325 g的雄性Sprague-Dawley大鼠皮下植入含0、10、25、50或100 mg DHEA或CORT的缓释微丸，持续3周。一般来说，随着类固醇激素水平的升高，体重和食物摄入量会下降。与DHEA治疗相反，接受50 mg和100 mg剂量CORT的大鼠胸腺和脾脏较轻，附睾和腹膜后脂肪垫比对照组重。用100 mg DHEA治疗的大鼠血清甘油三酯和脂质氢过氧化物水平降低，而用100 mg CORT治疗的大鼠与各自对照组相比，这些血脂水平更高。与DHEA治疗相反，CORT治疗导致肝脏脂质含量以及肝脏生脂酶葡萄糖-6-磷酸脱氢酶、苹果酸酶和脂肪酸合酶的比活性呈剂量依赖性增加。用100 mg DHEA治疗的大鼠血清IGF-1水平高于对照大鼠。相反，用100 mg CORT治疗的大鼠血清IGF-1水平较低，睾酮、孕酮和胰岛素的血清水平高于对照组。这些数据证明了皮质酮在大鼠中的生脂作用。相反，DHEA治疗降低了血清和肝脏脂质。此外，这些数据表明，使用植入物而非大剂量注射类固醇可能是研究这些类固醇对脂质代谢影响的更符合生理的方法。
