Han S, Kim H Y, Park K, Lee M S, Kim H J, Kim Y D
Department of Surgery, Inje University Sanggye Paik Hospital, Seoul, Korea.
J Surg Oncol. 1999 Jul;71(3):147-54. doi: 10.1002/(sici)1096-9098(199907)71:3<147::aid-jso3>3.0.co;2-5.
p27Kip1 is an inhibitor of cyclin-dependent kinases and is speculated to be a potential prognostic indicator in numerous human cancers. We investigated expression of p27Kip1 along with cyclin D1 in gastric cancer to estimate the clinical utility of p27Kip1.
Immunohistochemical assay for p27Kip1 and cyclin D1 proteins was performed in 64 patients with primary gastric cancer. Correlation between p27Kip1 expression and clinical-biological parameters including patient survival was analyzed.
p27Kip1 expression was suppressed in 40 (62.5%) of 64 gastric cancer patients and cyclin D1 was overexpressed in 22 (34.4%) out of 64. Expression of p27Kip1 was significantly reduced in poorly differentiated cancers (82.1%, 23/28; P = 0.015) and was also reduced in the tumors with high S-phase fraction (86.7%, 26/30) compared with tumors showing low S-phase fraction (41.2%, 14/34; P = 0.0002). Expression of p27Kip1 and cyclin D1 was inversely correlated (P = 0.021). In univariate analysis, extent of the disease (P < 0.001), expression of cyclin D1 (P = 0.0001), and reduced expression of p27Kip1 (P = 0. 0006), were statistically significant to predict patient's outcome, but depth of invasion (P = 0.008) and pathologic stage (P = 0.009) emerged as significant prognostic indicators in multivariate analysis.
Expression of p27Kip1 is closely linked with cell proliferation and differentiation of human gastric cancer. p27Kip1 seems to have potential as a prognostic marker in the management of gastric cancer patients.
p27Kip1是细胞周期蛋白依赖性激酶的一种抑制剂，据推测它是多种人类癌症潜在的预后指标。我们研究了p27Kip1和细胞周期蛋白D1在胃癌中的表达情况，以评估p27Kip1的临床应用价值。
对64例原发性胃癌患者进行p27Kip1和细胞周期蛋白D1蛋白的免疫组织化学检测。分析p27Kip1表达与包括患者生存情况在内的临床生物学参数之间的相关性。
64例胃癌患者中，40例（62.5%）p27Kip1表达受到抑制，64例中有22例（34.4%）细胞周期蛋白D1过表达。与低S期分数的肿瘤（41.2%，14/34；P = 0.0002）相比，在低分化癌中p27Kip1表达显著降低（82.1%，23/28；P = 0.015），在高S期分数的肿瘤中p27Kip1表达也降低（86.7%，26/30）。p27Kip1和细胞周期蛋白D1的表达呈负相关（P = 0.021）。单因素分析中，疾病范围（P < 0.001）、细胞周期蛋白D1的表达（P = 0.0001）以及p27Kip1表达降低（P = 0.0006）对预测患者预后具有统计学意义，但在多因素分析中，浸润深度（P = 0.008）和病理分期（P = 0.009）成为显著的预后指标。
p27Kip1的表达与人胃癌的细胞增殖和分化密切相关。p27Kip1在胃癌患者的管理中似乎有作为预后标志物的潜力。
