King L M, Brillard J P, Bakst M R, Donoghue A M
USDA, Agricultural Research Service, Livestock and Poultry Sciences Institute, Germplasm and Gamete Physiology Laboratory, Beltsville, Maryland 20705, USA.
Poult Sci. 1999 Jul;78(7):1044-7. doi: 10.1093/ps/78.7.1044.
This study was performed to determine whether intact sperm storage tubules (SST) could be successfully isolated from the uterovaginal junction (UVJ) mucosa of the turkey. Large White BUTA hens were inseminated and euthanatized 24 to 48 h later. Oviducts were excised, UVJ tissue removed, and SST were procured by enzymatic digestion. Recovered SST were intact and contained motile sperm. The sperm were oriented with their acrosomes pointed towards the distal end of the SST, and their long axes in parallel with the long axis of the tubule's lumen. This method for the isolation of intact SST can be readily applied for in vitro culture studies as well as for the extraction of DNA and RNA from the SST epithelium.
本研究旨在确定是否能够从火鸡的子宫阴道连接处（UVJ）黏膜成功分离出完整的精子储存小管（SST）。给大白BUTA母鸡输精，24至48小时后对其实施安乐死。切除输卵管，取出UVJ组织，通过酶消化法获取SST。回收的SST完整且含有活动精子。精子的顶体朝向SST的远端，其长轴与小管腔的长轴平行。这种分离完整SST的方法可轻易应用于体外培养研究以及从SST上皮细胞提取DNA和RNA。
