Wessman S J, Levings R L
USDA, APHIS, VS, Center for Veterinary Biologics-Laboratory, Ames, Iowa, USA.
Dev Biol Stand. 1999;99:3-8.
Infection with bovine viral diarrhoea virus (BVDV) and other viruses is frequent in the bovine population. In utero infection leads to virus and antibody contamination of foetal and other serum used in cell culture production. The use of contaminated cells for vaccine production may result in contaminated vaccines, which may lead to seroconversion or disease in the vaccinated animal. Contaminated serum or cell cultures may also interfere with the diagnosis of viral infections. Methods for the detection of BVDV and other viruses in serum, cell cultures, seed viruses and vaccines at the CVB-L, and the frequency of detection are described. Reasons for continued use of serum in cell culture production, and the risks of using serum, are discussed.
牛群中感染牛病毒性腹泻病毒（BVDV）和其他病毒的情况很常见。子宫内感染会导致用于细胞培养生产的胎儿血清和其他血清受到病毒和抗体污染。使用受污染的细胞生产疫苗可能会导致疫苗受污染，进而可能导致接种疫苗的动物发生血清转化或患病。受污染的血清或细胞培养物也可能干扰病毒感染的诊断。本文描述了在CVB-L检测血清、细胞培养物、种子病毒和疫苗中BVDV及其他病毒的方法以及检测频率。讨论了在细胞培养生产中持续使用血清的原因以及使用血清的风险。
