植物细胞的生长和分化可能涉及Rac活性的GAP调节。

Plant cell growth and differentiation may involve GAP regulation of Rac activity.

作者信息

Borg S, Pødenphant L, Jensen T J, Poulsen C

机构信息

Department of Structural and Molecular Biology, University of Aarhus, Aarhus C, Denmark.

出版信息

FEBS Lett. 1999 Jun 25;453(3):341-5. doi: 10.1016/s0014-5793(99)00750-4.

DOI:10.1016/s0014-5793(99)00750-4

PMID:10405172

Abstract

Two Rac GTPase cDNAs, LjRac1 and LjRac2, were identified in the legume Lotus japonicus. Two-hybrid screening with dominant-constitutive mutations in the two Rac GTPases target three plant cDNAs, LjRacGAP1, LjRacGAP2 and LjRacGAP3, that encode putative GTPase activating proteins of Rho-GTPase subfamily members. Employing Rac antiserum, purified recombinant LjRac GTPases and recombinant LjRacGAP1, for ligand overlay assays, in vitro GAP affinity assays and GTPase activation, we confirmed that eukaryote Rac/RacGAP interplay is conserved in plants. In this investigation we have developed some tools that can be used to characterize the role of enhanced LjRac2 expression in developing root nodules.

摘要

在豆科植物百脉根中鉴定出两个Rac GTP酶cDNA，即LjRac1和LjRac2。对这两个Rac GTP酶进行显性组成型突变的双杂交筛选，发现了三个植物cDNA，即LjRacGAP1、LjRacGAP2和LjRacGAP3，它们编码Rho-GTP酶亚家族成员的假定GTP酶激活蛋白。利用Rac抗血清、纯化的重组LjRac GTP酶和重组LjRacGAP1进行配体覆盖分析、体外GAP亲和力分析和GTP酶激活分析，我们证实了真核生物Rac/RacGAP相互作用在植物中是保守的。在本研究中，我们开发了一些工具，可用于表征增强的LjRac2表达在根瘤发育中的作用。