植物类Rac GTP酶被生长素激活并介导生长素响应基因的表达。
Plant Rac-like GTPases are activated by auxin and mediate auxin-responsive gene expression.
作者信息
Tao Li-zhen, Cheung Alice Y, Wu Hen-ming
机构信息
Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, Massachusetts 01003, USA.
出版信息
Plant Cell. 2002 Nov;14(11):2745-60. doi: 10.1105/tpc.006320.
Abstract
The auxin indole-3-acetic acid is a key plant hormone essential for a broad range of growth and developmental processes. Here, we show that auxin activates Rac-like GTPases (referred to as Rac/Rop GTPases), and they in turn stimulate auxin-responsive gene expression. In particular, we show that overexpressing a wild-type tobacco Rac/Rop GTPase, NtRac1, and its constitutively active mutant form activates auxin-responsive gene expression. On the other hand, overexpressing dominant-negative NtRac1 and Rac-negative regulators, or reducing the endogenous NtRac1 level, suppresses auxin-induced gene expression. Furthermore, overexpression of NtRac1 activity or suppression of its expression in transgenic seedlings induces phenotypes that are similar to auxin-related defects. Together, our results show that a subset of plant Rac/Rop GTPases functions in mediating the auxin signal to downstream responsive genes.
摘要
生长素吲哚 - 3 - 乙酸是一种关键的植物激素，对广泛的生长和发育过程至关重要。在此，我们表明生长素激活类Rac GTP酶（称为Rac/Rop GTP酶），而它们反过来又刺激生长素响应基因的表达。具体而言，我们发现过表达野生型烟草Rac/Rop GTP酶NtRac1及其组成型活性突变形式可激活生长素响应基因的表达。另一方面，过表达显性负性NtRac1和Rac负调控因子，或降低内源性NtRac1水平，会抑制生长素诱导的基因表达。此外，在转基因幼苗中过表达NtRac1活性或抑制其表达会诱导出与生长素相关缺陷相似的表型。总之，我们的结果表明，植物Rac/Rop GTP酶的一个子集在介导生长素信号至下游响应基因中发挥作用。
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本文引用的文献
1
Phenotypic characterization of a tobacco mutant impaired in auxin polar transport.生长素极性运输受损的烟草突变体的表型特征
Plant Cell Rep. 1997 Nov;17(1):32-38. doi: 10.1007/s002990050347.
2
A Mutation Altering Auxin Homeostasis and Plant Morphology in Arabidopsis.一个改变拟南芥生长素稳态和植物形态的突变
Plant Cell. 1995 Dec;7(12):2023-2037. doi: 10.1105/tpc.7.12.2023.
3
RopGAP4-dependent Rop GTPase rheostat control of Arabidopsis oxygen deprivation tolerance.依赖RopGAP4的Rop GTP酶对拟南芥缺氧耐受性的变阻器控制
Science. 2002 Jun 14;296(5575):2026-8. doi: 10.1126/science.1071505.
4
Small GTPases: versatile signaling switches in plants.小GTP酶：植物中多功能的信号开关
Plant Cell. 2002;14 Suppl(Suppl):S375-88. doi: 10.1105/tpc.001065.
5
Heterotrimeric and unconventional GTP binding proteins in plant cell signaling.植物细胞信号传导中的异源三聚体和非常规GTP结合蛋白。
Plant Cell. 2002;14 Suppl(Suppl):S355-73. doi: 10.1105/tpc.001792.
6
The ROP2 GTPase controls the formation of cortical fine F-actin and the early phase of directional cell expansion during Arabidopsis organogenesis.ROP2 GTP酶在拟南芥器官发生过程中控制皮层精细F-肌动蛋白的形成以及细胞定向扩张的早期阶段。
Plant Cell. 2002 Apr;14(4):777-94. doi: 10.1105/tpc.001537.
7
The Arabidopsis Rop2 GTPase is a positive regulator of both root hair initiation and tip growth.拟南芥Rop2 GTP酶是根毛起始和顶端生长的正向调节因子。
Plant Cell. 2002 Apr;14(4):763-76. doi: 10.1105/tpc.010359.
8
G protein-coupled receptor signalling and cross-talk: achieving rapidity and specificity.G蛋白偶联受体信号传导与相互作用：实现快速性与特异性
Cell Signal. 2002 May;14(5):397-405. doi: 10.1016/s0898-6568(01)00258-3.
9
AUX/IAA proteins are active repressors, and their stability and activity are modulated by auxin.AUX/IAA蛋白是活性阻遏物，其稳定性和活性受生长素调节。
Plant Cell. 2001 Dec;13(12):2809-22. doi: 10.1105/tpc.010289.
10
Auxin signaling: derepression through regulated proteolysis.生长素信号传导：通过调控蛋白水解实现去抑制
Dev Cell. 2001 Nov;1(5):595-604. doi: 10.1016/s1534-5807(01)00077-6.
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