通过改良碱裂解法和硅胶基质相结合的方法提取高质量质粒DNA。 - Suppr

Extraction of superior-quality plasmid DNA by a combination of modified alkaline lysis and silica matrix.

作者信息

Lakshmi R, Baskar V, Ranga U

机构信息

Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bangalore, 560 064, India.

出版信息

Anal Biochem. 1999 Jul 15;272(1):109-12. doi: 10.1006/abio.1999.4125.

DOI:10.1006/abio.1999.4125

PMID:10405301

Abstract

摘要

相似文献

Extraction of superior-quality plasmid DNA by a combination of modified alkaline lysis and silica matrix.

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引用本文的文献

SILEX: a fast and inexpensive high-quality DNA extraction method suitable for multiple sequencing platforms and recalcitrant plant species.

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Extraction of superior-quality plasmid DNA by a combination of modified alkaline lysis and silica matrix.

Anal Biochem. 1999 Jul 15;272(1):109-12. doi: 10.1006/abio.1999.4125.

Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction.

Appl Microbiol Biotechnol. 2009 Aug;84(1):199-204. doi: 10.1007/s00253-009-2088-3. Epub 2009 Jun 27.

Optimizing alkaline lysis for DNA plasmid recovery.

Biotechnol Appl Biochem. 2003 Jun;37(Pt 3):235-44. doi: 10.1042/BA20030002.

A novel method of plasmid isolation using laundry detergent.

Indian J Exp Biol. 2011 Jul;49(7):558-60.

Large-scale preparation of plasmid DNA by microwave lysis.

Biotechniques. 1995 Apr;18(4):554-5.

High-purity plasmid isolation using silica oxide.

Methods Mol Biol. 2003;235:83-7. doi: 10.1385/1-59259-409-3:83.

An automated process to extract plasmid DNA by alkaline lysis.

Appl Microbiol Biotechnol. 2007 Jul;75(5):1217-23. doi: 10.1007/s00253-007-0925-9. Epub 2007 Mar 27.

Isolation of plasmids from E. coli by alkaline lysis.

Methods Mol Biol. 2003;235:75-8. doi: 10.1385/1-59259-409-3:75.

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Biotechniques. 2000 Jan;28(1):107-9.

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Anal Biochem. 1999 Jun 15;271(1):89-91. doi: 10.1006/abio.1999.4106.

引用本文的文献

SILEX: a fast and inexpensive high-quality DNA extraction method suitable for multiple sequencing platforms and recalcitrant plant species.

Plant Methods. 2020 Aug 10;16:110. doi: 10.1186/s13007-020-00652-y. eCollection 2020.

Rapid Regeneration and Reuse of Silica Columns from PCR Purification and Gel Extraction Kits.

Sci Rep. 2018 Aug 27;8(1):12870. doi: 10.1038/s41598-018-30316-w.

Microbiological Sensing Technologies: A Review.

Bioengineering (Basel). 2018 Mar 2;5(1):20. doi: 10.3390/bioengineering5010020.

Extraction of superior-quality plasmid DNA by a combination of modified alkaline lysis and silica matrix.

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