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来自酿酒酵母二号染色体的12个开放阅读框的功能分析。

Functional analysis of 12 ORFs from Saccharomyces cerevisiae chromosome II.

作者信息

Rodríguez-Navarro S, Estruch F, Pérez-Ortín J E

机构信息

Departament de Bioquímica i Biología Molecular, Facultat de Ciències Biològiques, Universitat de València, Spain.

出版信息

Yeast. 1999 Jul;15(10B):913-9. doi: 10.1002/(SICI)1097-0061(199907)15:10B<913::AID-YEA386>3.0.CO;2-A.

DOI:10.1002/(SICI)1097-0061(199907)15:10B<913::AID-YEA386>3.0.CO;2-A
PMID:10407271
Abstract

Twelve different ORFs have been deleted from the right arm of Saccharomyces cerevisiae chromosome II; namely YBR193c, YBR194w, YBR197c, YBR198c, YBR201w, YBR203w, YBR207w, YBR209w, YBR210w, YBR211c, YBR217w and YBR228w. Tetrad analysis of heterozygous deletant strains revealed that YBR193c, YBR198c and YBR211c are essential genes for vegetative growth. No effects were detected in any of the haploid deletion mutants for the rest of the ORFs with respect to growth, gross morphology or mating.

摘要

酿酒酵母II号染色体右臂上的12个不同的开放阅读框(ORF)已被删除;即YBR193c、YBR194w、YBR197c、YBR198c、YBR201w、YBR203w、YBR207w、YBR209w、YBR210w、YBR211c、YBR217w和YBR228w。对杂合缺失菌株的四分体分析表明,YBR193c、YBR198c和YBR211c是营养生长所必需的基因。对于其余的开放阅读框,在任何单倍体缺失突变体中均未检测到对生长、总体形态或交配的影响。

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