Aubagnac J L, Enjalbal C, Drouot C, Combarieu R, Martinez J
UMR 5810, Laboratoire des Aminoacides, Peptides et Protéines, Université Montpellier II, Place E. Bataillon, 34095 Montpellier Cédex 05, France.
J Mass Spectrom. 1999 Jul;34(7):749-54. doi: 10.1002/(SICI)1096-9888(199907)34:7<749::AID-JMS828>3.0.CO;2-P.
Imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS) of solid-phase peptide syntheses carried out by the Merrifield and Sheppard strategies is described. Mixtures of resin beads mixed at random from batch syntheses or obtained in combinatorial chemistry by the mix and split technique, where each bead is functionalized by a unique peptide, were analyzed directly without any chemical cleavage of the growing chains to assess the nature of the growing structure on any bead of the mixture without its isolation.
描述了通过梅里菲尔德和谢泼德策略进行的固相肽合成的成像飞行时间二次离子质谱（TOF-SIMS）。对来自分批合成随机混合的树脂珠混合物或通过混合和拆分技术在组合化学中获得的混合物进行分析，其中每个珠子都被一种独特的肽功能化，无需对生长链进行任何化学切割即可直接分析，以评估混合物中任何珠子上生长结构的性质，而无需对其进行分离。
