Dhillon J K, Shivaraman N
National Environmental Engineering Research Institute (NEERI), Nehru Marg, Nagpur, India.
Can J Microbiol. 1999 Mar;45(3):201-8.
A Pseudomonas sp. (S1), isolated from soil by an enrichment technique was tested for its potential to degrade different cyanide compounds. Further, biodegradation/biotransformation of binary mixtures of the cyanide compounds by the culture was also studied. The results indicated that the culture could grow on the following nitriles by using them as carbon and nitrogen sources: acetonitrile, butyronitrile, acrylonitrile, adiponitrile, benzonitrile, glutaronitrile, phenylacetonitrile, and succinonitrile. Studies on the biodegradation of these cyanide compounds in binary mixtures showed that the presence of acrylonitrile or KCN delayed the degradation of acetonitrile in a mixture, while none of the other cyanide compounds affected the degradation of one another. The transformation products of the nitriles were their corresponding acids, and similarly, KCN was also directly transformed to formic acid. Studies on the transformation of these cyanide compounds showed that the rate of transformation of nitriles to their corresponding carboxylic acids was acrylonitrile > acetonitrile > adiponitrile > benzonitrile > KCN. This culture has the unique characteristic of transforming representatives of saturated aliphatic, aliphatic olefinic, aromatic, and aralkyl nitriles, as well as alkali cyanide, to their corresponding carboxylic acids.
通过富集技术从土壤中分离出的一株假单胞菌（S1），对其降解不同氰化物的潜力进行了测试。此外，还研究了该培养物对氰化物二元混合物的生物降解/生物转化。结果表明，该培养物可以利用以下腈类作为碳源和氮源进行生长：乙腈、丁腈、丙烯腈、己二腈、苯甲腈、戊二腈、苯乙腈和丁二腈。对这些氰化物二元混合物的生物降解研究表明，丙烯腈或氰化钾的存在会延迟混合物中乙腈的降解，而其他氰化物均不影响彼此的降解。腈类的转化产物是其相应的酸，同样，氰化钾也直接转化为甲酸。对这些氰化物转化的研究表明，腈类转化为其相应羧酸的速率为：丙烯腈＞乙腈＞己二腈＞苯甲腈＞氰化钾。该培养物具有将饱和脂肪族、脂肪族烯烃、芳香族和芳烷基腈以及碱金属氰化物的代表物转化为其相应羧酸的独特特性。
