质粒F和pSLT对FinP RNA的合成受DNA腺嘌呤甲基化调控。
Synthesis of FinP RNA by plasmids F and pSLT is regulated by DNA adenine methylation.
作者信息
Torreblanca J, Marqués S, Casadesús J
机构信息
Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Spain.
出版信息
Genetics. 1999 May;152(1):31-45. doi: 10.1093/genetics/152.1.31.
Abstract
DNA adenine methylase mutants of Salmonella typhimurium contain reduced amounts of FinP, an antisense RNA encoded by the virulence plasmid pSLT. Lowered FinP levels are detected in both Dam- FinO+ and Dam- FinO- backgrounds, suggesting that Dam methylation regulates FinP production rather than FinP half-life. Reduced amounts of F-encoded FinP RNA are likewise found in Dam- mutants of Escherichia coli. A consequence of FinP RNA scarcity in the absence of DNA adenine methylation is that Dam- mutants of both S. typhimurium and E. coli show elevated levels of F plasmid transfer. Inhibition of F fertility by the S. typhimurium virulence plasmid is also impaired in a Dam- background.
摘要
鼠伤寒沙门氏菌的DNA腺嘌呤甲基化酶突变体中，毒力质粒pSLT编码的反义RNA FinP含量降低。在Dam-FinO+和Dam-FinO-背景中均检测到FinP水平降低，这表明Dam甲基化调节FinP的产生而非FinP的半衰期。同样，在大肠杆菌的Dam-突变体中也发现F编码的FinP RNA含量减少。在缺乏DNA腺嘌呤甲基化的情况下，FinP RNA缺乏的一个后果是，鼠伤寒沙门氏菌和大肠杆菌的Dam-突变体均显示F质粒转移水平升高。鼠伤寒沙门氏菌毒力质粒对F育性的抑制在Dam-背景中也受到损害。
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