Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Apartado 1095, E-41080 Sevilla, Spain.
Institute of Microbiology and Infection, University of Birmingham, Birmingham B15 2TT, UK.
Cells. 2021 Apr 19;10(4):943. doi: 10.3390/cells10040943.
A bioinformatic search for LexA boxes, combined with transcriptomic detection of loci responsive to DNA damage, identified 48 members of the SOS regulon in the genome of serovar Typhimurium. Single cell analysis using fluorescent fusions revealed that heterogeneous expression is a common trait of SOS response genes, with formation of SOS and SOS subpopulations. Phenotypic cell variants formed in the absence of external DNA damage show gene expression patterns that are mainly determined by the position and the heterology index of the LexA box. SOS induction upon DNA damage produces SOS and SOS subpopulations that contain live and dead cells. The nature and concentration of the DNA damaging agent and the time of exposure are major factors that influence the population structure upon SOS induction. An analogy can thus be drawn between the SOS response and other bacterial stress responses that produce phenotypic cell variants.
生物信息学搜索 LexA 盒，结合对 DNA 损伤反应基因座的转录组检测，在血清型鼠伤寒沙门氏菌的基因组中鉴定出 48 个 SOS 调控基因。使用荧光融合的单细胞分析表明，异质表达是 SOS 反应基因的共同特征，形成 SOS 和 SOS 亚群。在没有外部 DNA 损伤的情况下形成的表型细胞变体显示出的基因表达模式主要由 LexA 盒的位置和异源性指数决定。DNA 损伤诱导的 SOS 诱导产生包含活细胞和死细胞的 SOS 和 SOS 亚群。DNA 损伤剂的性质和浓度以及暴露时间是影响 SOS 诱导时种群结构的主要因素。因此，可以将 SOS 反应与产生表型细胞变体的其他细菌应激反应进行类比。
