Nikai T, Suzuki Y, Komori Y, Sugihara H, Fox J W, Nonogaki T, Toyoda Y, Miwa I
Department of Microbiology, Faculty of Pharmacy, Meijo University, Nagoya, Japan.
J Nat Toxins. 1999 Jun;8(2):189-202.
A hemorrhagic toxin, designated Elegatoxin, was isolated from the venom of Trimeresurus elegans using HW-55, DEAE-Sephacel, CM-Cellulose and Mono S column chromatographies. The purified toxin was shown to be homogeneous by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, isoelectric electrophoresis, and Ouchterlony immunodiffusion. Elegatoxin has a molecular weight of 26,000 with an isoelectric point of 8.6. The toxin demonstrated both hemorrhagic and proteolytic activities. Hemorrhagic activity was inhibited by ethylenediaminetetraacetic acid (EDTA), ethyleneglycol-bis-(2-amino-ethylether)N,N'-tetraacetic acid (EGTA), o-phenanthroline, and N-bromosuccinimide, but not by amidinophenylmethanesulfonyl fluoride hydrochloride (APMSF). The minimum hemorrhagic dose was found to be 0.8 microgram/mouse. Elegatoxin possesses proteolytic activity as evidenced by hydrolyzing type IV collagen, actin and the A alpha, B beta, and gamma chains of bovine fibrinogen. This purified toxin contains 1 mol of zinc and 2 mols of calcium per mol of protein and a partial amino acid sequence was determined. The pathological and biochemical properties of Elegatoxin were investigated, and these results are reported in this paper.
从竹叶青蛇毒中分离出一种名为优雅毒素（Elegatoxin）的出血毒素，采用HW - 55、二乙氨基乙基纤维素（DEAE - Sephacel）、羧甲基纤维素（CM - Cellulose）和单磺酸柱色谱法。通过十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶电泳、等电聚焦电泳和免疫双扩散试验证明纯化后的毒素是均一的。优雅毒素分子量为26000，等电点为8.6。该毒素具有出血和蛋白水解活性。出血活性受到乙二胺四乙酸（EDTA）、乙二醇双（2 - 氨基乙醚）-N,N'-四乙酸（EGTA）、邻菲啰啉和N - 溴代琥珀酰亚胺抑制，但不受盐酸脒基苯基甲磺酰氟（APMSF）抑制。最小出血剂量为0.8微克/小鼠。优雅毒素具有蛋白水解活性，可水解IV型胶原、肌动蛋白以及牛纤维蛋白原的Aα、Bβ和γ链。每摩尔蛋白质该纯化毒素含有1摩尔锌和2摩尔钙，并测定了部分氨基酸序列。对优雅毒素的病理和生化特性进行了研究，本文报告了这些结果。
