Chen M F, Hsu H C, Liau C S, Lee Y T
Department of Internal Medicine (Cardiology), National Taiwan University Hospital, Taipei.
Prostaglandins Other Lipid Mediat. 1999 May;57(2-3):99-111. doi: 10.1016/s0090-6980(98)00075-6.
The role of vitamin E on the anti-atherosclerotic effect of fish oil in diet-induced hypercholesterolemic rabbits was studied in male New Zealand white rabbits. The animals were randomly divided into six groups of 14 each according to the chow given them. Group I, control, rabbits were fed regular laboratory rabbit chow. In addition to the regular chow, Group II rabbits were fed a high (1%) cholesterol-enriched diet. Group III had the same diet as Group II plus 450 mg vitamin E/1000 g chow. Group IV also had the same diet as Group II plus 10% fish oil, but without vitamin E. Group V's diet contained 1% cholesterol, 10% fish oil, and 450 mg vitamin E/1000 g chow. Group VI had the same diet as Group V, but with 150 mg vitamin E/1000 g chow. At the end of a 6-week feeding, the ascending aorta from seven rabbits from each group was harvested for the analysis of prostanoid production, thiobarbituric acid reactive substances (TBARS), superoxide dismutase activity, and cholesterol and vitamin E levels; the same tissue from the other seven rabbits from each group was obtained for the delineation of atherosclerotic lesions by planimetry after the Sudan IV stain. The high cholesterol diet-treated rabbits had worse prostanoid synthesis and higher TBARS levels, which paralleled the severity of the atherosclerosis. Vitamin E or fish oil supplementation in a high cholesterol diet had beneficial prostanoid production, reduced aortic TBARS levels, and attenuated atherosclerotic lesions; these effects were potentiated when vitamin E and fish oil were combined together. The atherosclerotic lesion reduction in rabbits treated with high cholesterol plus fish oil and 450 mg vitamin E/1000 g chow reached statistical significance (P < 0.05) compared to high cholesterol and the same dose of vitamin E-treated rabbits. The rabbits treated with high cholesterol plus fish oil, but without vitamin E or with 150 mg vitamin E/1000 g chow showed an increased plasma creatine kinase activity at 4 (P < 0.01 and 0.05, respectively) and 6 (P < 0.001 and 0.01, respectively) week of feeding. These results suggest that vitamin E and/or fish oil attenuate atherosclerosis in high cholesterol-fed rabbits; vitamin E and fish oil potentiated the effect of each other. Furthermore, without sufficient vitamin E supplementation, rabbits treated with high cholesterol plus fish oil will show an elevation of plasma creatine kinase activity.
在雄性新西兰白兔中研究了维生素E对鱼油在饮食诱导的高胆固醇血症兔中的抗动脉粥样硬化作用。根据给予动物的食物，将它们随机分为六组，每组14只。第一组为对照组，兔子喂食常规实验室兔粮。除常规兔粮外，第二组兔子喂食高胆固醇（1%）强化饮食。第三组的饮食与第二组相同，但每1000克兔粮中添加450毫克维生素E。第四组的饮食也与第二组相同，但添加10%的鱼油，不含维生素E。第五组的饮食含有1%的胆固醇、10%的鱼油和每1000克兔粮中450毫克维生素E。第六组的饮食与第五组相同，但每1000克兔粮中含有150毫克维生素E。在6周喂养期结束时，每组7只兔子的升主动脉被采集用于分析前列腺素生成、硫代巴比妥酸反应性物质（TBARS）、超氧化物歧化酶活性以及胆固醇和维生素E水平；每组另外7只兔子的相同组织在苏丹IV染色后通过平面测量法用于描绘动脉粥样硬化病变。高胆固醇饮食处理的兔子前列腺素合成更差，TBARS水平更高，这与动脉粥样硬化的严重程度平行。在高胆固醇饮食中补充维生素E或鱼油具有有益的前列腺素生成，降低主动脉TBARS水平，并减轻动脉粥样硬化病变；当维生素E和鱼油联合使用时，这些作用会增强。与高胆固醇和相同剂量维生素E处理的兔子相比，高胆固醇加鱼油和每1000克兔粮中45mg维生素E处理的兔子动脉粥样硬化病变减少具有统计学意义（P<0.05）。高胆固醇加鱼油但不添加维生素E或每1000克兔粮中添加150毫克维生素E处理的兔子在喂养4周（分别为P<0.01和0.05）和6周（分别为P<0.001和0.01）时血浆肌酸激酶活性增加。这些结果表明，维生素E和/或鱼油可减轻高胆固醇喂养兔的动脉粥样硬化；维生素E和鱼油相互增强作用。此外，在没有足够维生素E补充的情况下，高胆固醇加鱼油处理的兔子会出现血浆肌酸激酶活性升高。
