Matsui K, Oda Y, Nakata H, Yoshimura T
Drug Evaluation Research Laboratories, Eisai Co., Ltd., Ibaraki, Japan.
J Chromatogr B Biomed Sci Appl. 1999 Jun 11;729(1-2):147-55. doi: 10.1016/s0378-4347(99)00145-0.
A rapid, sensitive and enantioselective LC-MS-MS method using deuterium-labeled internal standard was developed and evaluated for the simultaneous quantitative determination of donepezil enantiomers in human plasma without interconversion during clean-up process and measurement. The use of an avidin column allowed the separation of donepezil enantiomers, which were specifically detected by MS-MS without interference from its metabolites and plasma constituents. Evaluation of this assay method shows that samples can be assayed with acceptable accuracy and precision within the range from 0.0206 ng/ml to 51.6 ng/ml for both R-donepezil and S-donepezil. This analytical method was applied to the simultaneous quantitation of donepezil enantiomers in human plasma.
建立了一种使用氘代内标的快速、灵敏且对映体选择性的液相色谱-串联质谱法，用于同时定量测定人血浆中多奈哌齐对映体，在净化过程和测量期间不会发生相互转化。使用抗生物素蛋白柱可分离多奈哌齐对映体，通过串联质谱法对其进行特异性检测，不受其代谢产物和血浆成分的干扰。该分析方法的评估表明，对于R-多奈哌齐和S-多奈哌齐，在0.0206 ng/ml至51.6 ng/ml范围内，样品测定的准确度和精密度均可接受。该分析方法应用于同时定量测定人血浆中多奈哌齐对映体。
