Valdez J S, Martin D K, Mayersohn M
Phoenix Police Department Laboratory Bureau, AZ 85003, USA.
J Chromatogr B Biomed Sci Appl. 1999 Jun 11;729(1-2):163-71. doi: 10.1016/s0378-4347(99)00161-9.
Analytical methods using gas chromatography-flame ionization detection (GC-FID) for the quantitation of camphor and menthol and GC-MS for the quantitation of methyl salicylate have been developed for measurement of low concentrations from human plasma. Anethole serves as the internal standard for camphor and menthol and ethyl salicylate serves as the internal standard for methyl salicylate. Plasma samples undergo multiple, sequential extractions with hexane in order to provide optimal recovery. For menthol and camphor, the extracting solvent is reduced in volume and directly injected onto a capillary column (Simplicity-WAX). Extracted methyl salicylate is derivatized with BSTFA prior to injection onto a capillary column (Simplicity-5). Between-day variation (% RSD) at 5 ng/ml varies from 6.2% for methyl salicylate to 13.5% for camphor. The limit of detection for each analyte is 1 ng/ml and the limit of quantitation is 5 ng/ml. These analytical methods have been used in a clinical study to assess exposure from dermally applied patches containing the three compounds.
已开发出使用气相色谱 - 火焰离子化检测（GC - FID）定量樟脑和薄荷醇以及使用气相色谱 - 质谱联用（GC - MS）定量水杨酸甲酯的分析方法，用于测量人血浆中的低浓度物质。茴香脑用作樟脑和薄荷醇的内标，水杨酸乙酯用作水杨酸甲酯的内标。血浆样品用己烷进行多次连续萃取，以实现最佳回收率。对于薄荷醇和樟脑，萃取溶剂体积减少后直接注入毛细管柱（Simplicity - WAX）。萃取的水杨酸甲酯在注入毛细管柱（Simplicity - 5）之前用BSTFA进行衍生化。5 ng/ml时的日间变化（%RSD），水杨酸甲酯为6.2%，樟脑为13.5%。每种分析物的检测限为1 ng/ml，定量限为5 ng/ml。这些分析方法已用于一项临床研究，以评估含有这三种化合物的皮肤贴片的暴露情况。
