Everhart E T, Shwonek P, Jacob P, Rowbotham M C, Jones R T
Drug Dependence Research Center, Department of Psychiatry, University of California at San Francisco, 94143, USA.
J Chromatogr B Biomed Sci Appl. 1999 Jun 11;729(1-2):173-81. doi: 10.1016/s0378-4347(99)00160-7.
A gas chromatographic method for the determination of levorphanol in human plasma is described. The method utilizes extractive alkylation with tetrabutylammonium cation as the phase-transfer catalyst and pentafluorobenzyl bromide as the alkylating agent, and employs a structural analog, d-3-hydroxy-N-ethylmorphinan, as the internal standard. The pentafluorobenzyl ethers formed are separated by capillary gas chromatography and detected by electron capture. The method has good precision and accuracy for concentrations ranging from 0.25 ng/ml to 100 ng/ml and has been used to measure plasma concentrations as part of a study to evaluate the management of chronic neuropathic pain with levorphanol.
本文描述了一种测定人血浆中左啡诺的气相色谱法。该方法利用四丁基铵阳离子作为相转移催化剂、五氟苄基溴作为烷基化剂进行萃取烷基化，并采用结构类似物d-3-羟基-N-乙基吗啡喃作为内标。形成的五氟苄基醚通过毛细管气相色谱法分离，并用电子捕获法检测。该方法对于0.25 ng/ml至100 ng/ml的浓度具有良好的精密度和准确度，并且已作为评估左啡诺治疗慢性神经性疼痛管理的研究的一部分，用于测量血浆浓度。
