Bot A, Shearer M, Bot S, Woods C, Limmer J, Kennedy R, Casares S, Bona C
Alliance Pharmaceutical Corp., San Diego, California, USA.
Viral Immunol. 1999;12(2):91-6. doi: 10.1089/vim.1999.12.91.
Previous studies showed that DNA immunization of newborn mice with plasmids expressing influenza virus antigens induced protective immunity. We have now extended the study of neonatal responsiveness to DNA vaccines to nonhuman primates. Baboons immunized as neonates with plasmids expressing type A influenza virus hemagglutinin (HA) and nucleoprotein (NP) in doses ranging from 40 microg to 1 mg per plasmid per dose developed virus-specific humoral responses. The titer and kinetics of appearance of virus-specific IgG antibodies were dose dependent. Specific antibodies were detected by enzyme-linked immunosorbent assay (ELISA) as early as 1 month after birth in baboons immunized with the highest and intermediate doses of vaccine. Virus-neutralizing antibodies were detected in the group of baboons immunized with the highest dose. The specificity of virus-neutralizing antibodies was found to be directed against homologous determinants of HA; however, the IgG antibodies also cross-reacted with HA of a drift variant. Thus, DNA vaccination of newborn baboons with a prototype vaccine against influenza virus resulted in induction of specific humoral immunity.
先前的研究表明，用表达流感病毒抗原的质粒对新生小鼠进行DNA免疫可诱导保护性免疫。我们现在已将对DNA疫苗的新生儿反应性研究扩展到非人灵长类动物。用每剂质粒中表达甲型流感病毒血凝素（HA）和核蛋白（NP）的剂量范围为40微克至1毫克的质粒对新生狒狒进行免疫，可产生病毒特异性体液反应。病毒特异性IgG抗体出现的滴度和动力学呈剂量依赖性。在用最高和中等剂量疫苗免疫的狒狒中，早在出生后1个月就通过酶联免疫吸附测定（ELISA）检测到特异性抗体。在用最高剂量免疫的狒狒组中检测到病毒中和抗体。发现病毒中和抗体的特异性针对HA的同源决定簇；然而，IgG抗体也与一个漂移变异株的HA发生交叉反应。因此，用一种抗流感病毒原型疫苗对新生狒狒进行DNA疫苗接种可诱导特异性体液免疫。
