Ren Z, Pang G, Musicka M, Dunkley M, Batey R, Beagley K, Clancy R
Discipline of Pathology, Faculty of Medicine and Health Sciences, University of Newcastle, New South Wales, Australia.
Microbios. 1999;97(388):153-63.
Controversy exists as to whether the coccoid form of Helicobacter pylori can exist in a viable form. Conversion of helical to coccoid morphology occurs in culture over several days. In this study, the morphology was correlated with parameters of genetic integrity in the reference NCTC 11637 strain over 21 days of culture. The capacity to regrow colonies of helical form was demonstrated from a culture where the coccoid form constituted up to 95% and negligible urease activity could be detected. Urease enzyme activity and its mRNA decreased between day 0 and 10 while 26 kD mRNA and 16S rRNA were expressed unchanged for up to 14 and 21 days of culture, respectively. Expression of mRNA for the Cag A gene behaved in a similar fashion to that of urease. No evidence of DNA fragmentation was detected. These data suggest that a viable form of non-urease producing H. pylori exists after short to intermediate culture and that some if not all of these viable bacteria have coccoid morphology.
关于幽门螺杆菌的球形形式是否能以活的形态存在存在争议。在培养过程中，螺旋形向球形形态的转变会在数天内发生。在本研究中，对参考菌株NCTC 11637在21天的培养过程中，形态与遗传完整性参数进行了关联。从球形形式占比高达95%且检测到的尿素酶活性可忽略不计的培养物中，证明了螺旋形菌落的再生长能力。在第0天到第10天之间，尿素酶活性及其mRNA下降，而26 kD mRNA和16S rRNA分别在长达14天和21天的培养过程中表达不变。Cag A基因的mRNA表达与尿素酶的表达情况类似。未检测到DNA片段化的证据。这些数据表明，在短期至中期培养后，存在一种不产生尿素酶的活的幽门螺杆菌形式，并且这些活细菌中一些（如果不是全部）具有球形形态。
